A glimpse into the clinical proteome of human malaria parasites Plasmodium falciparum and Plasmodium vivax

Pragyan Acharya, Rani Pallavi, Syama Chandran, Harshini Chakravarti, Sheetal Middha, Jyoti Acharya, Sanjay Kochar, Dhanpat Kochar, Amit Subudhi, Arun P. Boopathi, Shilpi Garg, Ashis Das, Utpal Tatu

Research output: Contribution to journalArticle

Abstract

Malaria causes a worldwide annual mortality of about a million people. Rapidly evolving drugresistant species of the parasite have created a pressing need for the identification of new drug targets and vaccine candidates. By developing fractionation protocols to enrich parasites from low-parasitemia patient samples, we have carried out the first ever proteomics analysis of clinical isolates of early stages of Plasmodium falciparum (Pf) and P. vivax. Patient-derived malarial parasites were directly processed and analyzed using shotgun proteomics approach using high-sensitivity MS for protein identification. Our study revealed about 100 parasitecoded gene products that included many known drug targets such as Pf hypoxanthine guanine phosphoribosyl transferase, Pf L-lactate dehydrogenase, and Plasmepsins. In addition, our study reports the expression of several parasite proteins in clinical ring stages that have never been reported in the ring stages of the laboratory-cultivated parasite strain. This proof-of-principle study represents a noteworthy step forward in our understanding of pathways elaborated by the parasite within the malaria patient and will pave the way towards identification of new drug and vaccine targets that can aid malaria therapy.

Original languageEnglish
Pages (from-to)1314-1325
Number of pages12
JournalProteomics - Clinical Applications
Volume3
Issue number11
DOIs
Publication statusPublished - Dec 2009

Keywords

  • Malaria
  • MS
  • Patient sample
  • Plasmodium

ASJC Scopus subject areas

  • Clinical Biochemistry

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