A growth factor different from IL-6 promotes the growth of Epstein-Barr virus-transformed cells at low cell density

Claudia Negri; Antonella Cerino; Roberto Chiesa e; Giulia C B Astaldi Ricotti

doi:10.1007/BF00736830

A growth factor different from IL-6 promotes the growth of Epstein-Barr virus-transformed cells at low cell density

Claudia Negri, Antonella Cerino, Roberto Chiesa e, Giulia C B Astaldi Ricotti

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

Interleukin-6 (IL-6) was recently described to support the growth of Epstein-Barr (EBV) transformed lymphocytes. However no effect was reported on cloning of EBV transformed lymphocytes under limiting dilution conditions. In this paper we demonstrate that the Supernatant of Human Endothelial Cells (HECS) contains a factor(s) different from IL-6 which induces high cloning efficiency of EBV transformed cell lines, cultured under limiting dilution conditions. Furthermore HECS is superior to IL-6 in all the passages of EBV lymphocytes transformation.

Original language	English
Pages (from-to)	132-133
Number of pages	2
Journal	Cytotechnology
Volume	5
Issue number	1 Supplement
DOIs	https://doi.org/10.1007/BF00736830
Publication status	Published - Feb 1991

ASJC Scopus subject areas

Clinical Biochemistry
Cell Biology
Biotechnology

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10.1007/BF00736830

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title = "A growth factor different from IL-6 promotes the growth of Epstein-Barr virus-transformed cells at low cell density",

abstract = "Interleukin-6 (IL-6) was recently described to support the growth of Epstein-Barr (EBV) transformed lymphocytes. However no effect was reported on cloning of EBV transformed lymphocytes under limiting dilution conditions. In this paper we demonstrate that the Supernatant of Human Endothelial Cells (HECS) contains a factor(s) different from IL-6 which induces high cloning efficiency of EBV transformed cell lines, cultured under limiting dilution conditions. Furthermore HECS is superior to IL-6 in all the passages of EBV lymphocytes transformation.",

author = "Claudia Negri and Antonella Cerino and {Chiesa e}, Roberto and {Astaldi Ricotti}, {Giulia C B}",

year = "1991",

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AU - Negri, Claudia

AU - Cerino, Antonella

AU - Chiesa e, Roberto

AU - Astaldi Ricotti, Giulia C B

PY - 1991/2

Y1 - 1991/2

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AB - Interleukin-6 (IL-6) was recently described to support the growth of Epstein-Barr (EBV) transformed lymphocytes. However no effect was reported on cloning of EBV transformed lymphocytes under limiting dilution conditions. In this paper we demonstrate that the Supernatant of Human Endothelial Cells (HECS) contains a factor(s) different from IL-6 which induces high cloning efficiency of EBV transformed cell lines, cultured under limiting dilution conditions. Furthermore HECS is superior to IL-6 in all the passages of EBV lymphocytes transformation.

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A growth factor different from IL-6 promotes the growth of Epstein-Barr virus-transformed cells at low cell density

Abstract

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