A guinea pig model of mesial temporal lobe epilepsy following nonconvulsive status epilepticus induced by unilateral intrahippocampal injection of kainic acid

Giovanni Carriero, Salvatore Arcieri, Alessandro Cattalini, Laura Corsi, Vadym Gnatkovsky, Marco De Curtis

Research output: Contribution to journalArticle

Abstract

Purpose: Models of temporal lobe epilepsy are commonly utilized to study focal epileptogenesis and ictogenesis. The criteria that define animal models representative of human mesial temporal lobe may vary in different laboratories. We describe herein a focal epilepsy model of mesial temporal (hippocampal) origin that relies on the analysis of interictal and ictal electroencephalography (EEG) patterns and on their correlation with seizure symptoms and neuropathologic findings. The study is based on guinea pigs, a species seldom utilized to develop chronic epilepsy models. Methods: Young adult guinea pigs were bilaterally implanted under isoflurane anesthesia with epidural electrodes over somatosensory cortex and depth electrodes in CA1 hippocampal region. A stainless steel guide cannula was positioned unilaterally in the right dorsal hippocampus to inject 1 μl of 0.9% NaCl solution containing 1 μg kainic acid (KA). One week after surgery, continuous 24 h/day video-EEG monitoring was performed 48 h before and every other week after KA injection, for no

Original languageEnglish
Pages (from-to)1917-1927
Number of pages11
JournalEpilepsia
Volume53
Issue number11
DOIs
Publication statusPublished - Nov 2012

Keywords

  • Guinea pig
  • Intrahippocampal kainic acid injection
  • Mesial temporal lobe epilepsy model

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

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