A human alphoid DNA clone from the EcoRI dimeric family: Genomic and internal organization and chromosomal assignment

A. Baldini; D. I. Smith; M. Rocchi; O. J. Miller; D. A. Miller

doi:10.1016/0888-7543(89)90124-9

A human alphoid DNA clone from the EcoRI dimeric family: Genomic and internal organization and chromosomal assignment

A. Baldini, D. I. Smith, M. Rocchi, O. J. Miller, D. A. Miller

www.neuromed.it

Research output: Contribution to journal › Article › peer-review

Abstract

We isolated an alpha satellite DNA clone (pC1.8), 17 kb long, which is composed exclusively of tandemly repeated 340-bp EcoRI fragments. Hybridization studies using 37 random EcoRI dimers subcloned from pC1.8 showed that they are heterogeneous. The sequence of 5 dimers, 3 of them adjacent, confirmed this observation and showed that the heterogeneity is more accentuated among the second monomers. The chromosomal assignment under high stringency conditions showed that this alphoid subset is located on chromosomes 1, 5, and 19. No conditions that eliminate the hybridization on any one of those chromosomes were found. This suggests that, in contrast to many other chromosome-specific alpha satellite subsets, the single chromosome subsets of this family are virtually indistinguishable by hybridization techniques.

Original language	English
Pages (from-to)	822-828
Number of pages	7
Journal	Genomics
Volume	5
Issue number	4
DOIs	https://doi.org/10.1016/0888-7543(89)90124-9
Publication status	Published - 1989

ASJC Scopus subject areas

Genetics

Access to Document

10.1016/0888-7543(89)90124-9

Cite this

@article{9fd979fb7ff344aba2f80a6ddf54209b,

title = "A human alphoid DNA clone from the EcoRI dimeric family: Genomic and internal organization and chromosomal assignment",

abstract = "We isolated an alpha satellite DNA clone (pC1.8), 17 kb long, which is composed exclusively of tandemly repeated 340-bp EcoRI fragments. Hybridization studies using 37 random EcoRI dimers subcloned from pC1.8 showed that they are heterogeneous. The sequence of 5 dimers, 3 of them adjacent, confirmed this observation and showed that the heterogeneity is more accentuated among the second monomers. The chromosomal assignment under high stringency conditions showed that this alphoid subset is located on chromosomes 1, 5, and 19. No conditions that eliminate the hybridization on any one of those chromosomes were found. This suggests that, in contrast to many other chromosome-specific alpha satellite subsets, the single chromosome subsets of this family are virtually indistinguishable by hybridization techniques.",

author = "A. Baldini and Smith, {D. I.} and M. Rocchi and Miller, {O. J.} and Miller, {D. A.}",

year = "1989",

doi = "10.1016/0888-7543(89)90124-9",

language = "English",

volume = "5",

pages = "822--828",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "4",

}

TY - JOUR

T1 - A human alphoid DNA clone from the EcoRI dimeric family

T2 - Genomic and internal organization and chromosomal assignment

AU - Baldini, A.

AU - Smith, D. I.

AU - Rocchi, M.

AU - Miller, O. J.

AU - Miller, D. A.

PY - 1989

Y1 - 1989

N2 - We isolated an alpha satellite DNA clone (pC1.8), 17 kb long, which is composed exclusively of tandemly repeated 340-bp EcoRI fragments. Hybridization studies using 37 random EcoRI dimers subcloned from pC1.8 showed that they are heterogeneous. The sequence of 5 dimers, 3 of them adjacent, confirmed this observation and showed that the heterogeneity is more accentuated among the second monomers. The chromosomal assignment under high stringency conditions showed that this alphoid subset is located on chromosomes 1, 5, and 19. No conditions that eliminate the hybridization on any one of those chromosomes were found. This suggests that, in contrast to many other chromosome-specific alpha satellite subsets, the single chromosome subsets of this family are virtually indistinguishable by hybridization techniques.

AB - We isolated an alpha satellite DNA clone (pC1.8), 17 kb long, which is composed exclusively of tandemly repeated 340-bp EcoRI fragments. Hybridization studies using 37 random EcoRI dimers subcloned from pC1.8 showed that they are heterogeneous. The sequence of 5 dimers, 3 of them adjacent, confirmed this observation and showed that the heterogeneity is more accentuated among the second monomers. The chromosomal assignment under high stringency conditions showed that this alphoid subset is located on chromosomes 1, 5, and 19. No conditions that eliminate the hybridization on any one of those chromosomes were found. This suggests that, in contrast to many other chromosome-specific alpha satellite subsets, the single chromosome subsets of this family are virtually indistinguishable by hybridization techniques.

UR - http://www.scopus.com/inward/record.url?scp=0024758528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024758528&partnerID=8YFLogxK

U2 - 10.1016/0888-7543(89)90124-9

DO - 10.1016/0888-7543(89)90124-9

M3 - Article

C2 - 2591965

AN - SCOPUS:0024758528

VL - 5

SP - 822

EP - 828

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 4

ER -

A human alphoid DNA clone from the EcoRI dimeric family: Genomic and internal organization and chromosomal assignment

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this