A human myeloma immunoglobulin G binding four moles of calcium associated with asymptomatic hypercalcemia

Giampaolo Merlini, Lorraine A. Fitzpatrick, Ethel S. Siris, John P. Bilezikian, Steven Birken, Sherman Beychok, Elliott F. Osserman

Research output: Contribution to journalArticle

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Abstract

A calcium-binding immunoglobulin G (IgGIλRUP) was identified in the serum of a patient with multiple myeloma, asymptomatic hypercalcemia, and a normal ionized serum calcium. Calcium binding by IgGRUP was confirmed by two-dimensional electrophoresis with calcium-45 and equilibrium dialysis. Amino acid analyses indicated an unusually high number of glutamic (or glutamine) residues in the L chain and Fab fragment but no detectable γ-carboxyglutamic acid. As determined by equilibrium dialysis with45Ca, the intact IgGRUP and its Fab fragments bound calcium at an optimum pH of 7.4. There was minimal binding of calcium to H chains and no binding by L chains or the Fc fragment. Recombination of H and L chains partially restored the binding activity. By Scatchard analysis, the binding affinity (Kd) of IgGRUP was 1.7×10-3M and the binding capacity was 4 mol of calcium/mol of IgG. The binding of 4 mol of calcium/mol of IgG is twice that reported previously for two other calcium-binding myeloma proteins and suggests unique properties of IgGRUP.

Original languageEnglish
Pages (from-to)185-196
Number of pages12
JournalJournal of Clinical Immunology
Volume4
Issue number3
DOIs
Publication statusPublished - May 1984

Fingerprint

Hypercalcemia
Immunoglobulin G
Calcium
Immunoglobulin Fab Fragments
Dialysis
Immunoglobulin Fc Fragments
Myeloma Proteins
Calcium-Binding Proteins
myeloma immunoglobulins
Glutamine
Serum
Multiple Myeloma
Genetic Recombination
Electrophoresis
Amino Acids
Acids

Keywords

  • calcium binding
  • IgG
  • Myeloma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Merlini, G., Fitzpatrick, L. A., Siris, E. S., Bilezikian, J. P., Birken, S., Beychok, S., & Osserman, E. F. (1984). A human myeloma immunoglobulin G binding four moles of calcium associated with asymptomatic hypercalcemia. Journal of Clinical Immunology, 4(3), 185-196. https://doi.org/10.1007/BF00914965

A human myeloma immunoglobulin G binding four moles of calcium associated with asymptomatic hypercalcemia. / Merlini, Giampaolo; Fitzpatrick, Lorraine A.; Siris, Ethel S.; Bilezikian, John P.; Birken, Steven; Beychok, Sherman; Osserman, Elliott F.

In: Journal of Clinical Immunology, Vol. 4, No. 3, 05.1984, p. 185-196.

Research output: Contribution to journalArticle

Merlini, G, Fitzpatrick, LA, Siris, ES, Bilezikian, JP, Birken, S, Beychok, S & Osserman, EF 1984, 'A human myeloma immunoglobulin G binding four moles of calcium associated with asymptomatic hypercalcemia', Journal of Clinical Immunology, vol. 4, no. 3, pp. 185-196. https://doi.org/10.1007/BF00914965
Merlini, Giampaolo ; Fitzpatrick, Lorraine A. ; Siris, Ethel S. ; Bilezikian, John P. ; Birken, Steven ; Beychok, Sherman ; Osserman, Elliott F. / A human myeloma immunoglobulin G binding four moles of calcium associated with asymptomatic hypercalcemia. In: Journal of Clinical Immunology. 1984 ; Vol. 4, No. 3. pp. 185-196.
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