Abstract
A calcium-binding immunoglobulin G (IgGIλRUP) was identified in the serum of a patient with multiple myeloma, asymptomatic hypercalcemia, and a normal ionized serum calcium. Calcium binding by IgGRUP was confirmed by two-dimensional electrophoresis with calcium-45 and equilibrium dialysis. Amino acid analyses indicated an unusually high number of glutamic (or glutamine) residues in the L chain and Fab fragment but no detectable γ-carboxyglutamic acid. As determined by equilibrium dialysis with45Ca, the intact IgGRUP and its Fab fragments bound calcium at an optimum pH of 7.4. There was minimal binding of calcium to H chains and no binding by L chains or the Fc fragment. Recombination of H and L chains partially restored the binding activity. By Scatchard analysis, the binding affinity (Kd) of IgGRUP was 1.7×10-3M and the binding capacity was 4 mol of calcium/mol of IgG. The binding of 4 mol of calcium/mol of IgG is twice that reported previously for two other calcium-binding myeloma proteins and suggests unique properties of IgGRUP.
| Original language | English |
|---|---|
| Pages (from-to) | 185-196 |
| Number of pages | 12 |
| Journal | Journal of Clinical Immunology |
| Volume | 4 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 1984 |
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Keywords
- calcium binding
- IgG
- Myeloma
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
A human myeloma immunoglobulin G binding four moles of calcium associated with asymptomatic hypercalcemia. / Merlini, Giampaolo; Fitzpatrick, Lorraine A.; Siris, Ethel S.; Bilezikian, John P.; Birken, Steven; Beychok, Sherman; Osserman, Elliott F.
In: Journal of Clinical Immunology, Vol. 4, No. 3, 05.1984, p. 185-196.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A human myeloma immunoglobulin G binding four moles of calcium associated with asymptomatic hypercalcemia
AU - Merlini, Giampaolo
AU - Fitzpatrick, Lorraine A.
AU - Siris, Ethel S.
AU - Bilezikian, John P.
AU - Birken, Steven
AU - Beychok, Sherman
AU - Osserman, Elliott F.
PY - 1984/5
Y1 - 1984/5
N2 - A calcium-binding immunoglobulin G (IgGIλRUP) was identified in the serum of a patient with multiple myeloma, asymptomatic hypercalcemia, and a normal ionized serum calcium. Calcium binding by IgGRUP was confirmed by two-dimensional electrophoresis with calcium-45 and equilibrium dialysis. Amino acid analyses indicated an unusually high number of glutamic (or glutamine) residues in the L chain and Fab fragment but no detectable γ-carboxyglutamic acid. As determined by equilibrium dialysis with45Ca, the intact IgGRUP and its Fab fragments bound calcium at an optimum pH of 7.4. There was minimal binding of calcium to H chains and no binding by L chains or the Fc fragment. Recombination of H and L chains partially restored the binding activity. By Scatchard analysis, the binding affinity (Kd) of IgGRUP was 1.7×10-3M and the binding capacity was 4 mol of calcium/mol of IgG. The binding of 4 mol of calcium/mol of IgG is twice that reported previously for two other calcium-binding myeloma proteins and suggests unique properties of IgGRUP.
AB - A calcium-binding immunoglobulin G (IgGIλRUP) was identified in the serum of a patient with multiple myeloma, asymptomatic hypercalcemia, and a normal ionized serum calcium. Calcium binding by IgGRUP was confirmed by two-dimensional electrophoresis with calcium-45 and equilibrium dialysis. Amino acid analyses indicated an unusually high number of glutamic (or glutamine) residues in the L chain and Fab fragment but no detectable γ-carboxyglutamic acid. As determined by equilibrium dialysis with45Ca, the intact IgGRUP and its Fab fragments bound calcium at an optimum pH of 7.4. There was minimal binding of calcium to H chains and no binding by L chains or the Fc fragment. Recombination of H and L chains partially restored the binding activity. By Scatchard analysis, the binding affinity (Kd) of IgGRUP was 1.7×10-3M and the binding capacity was 4 mol of calcium/mol of IgG. The binding of 4 mol of calcium/mol of IgG is twice that reported previously for two other calcium-binding myeloma proteins and suggests unique properties of IgGRUP.
KW - calcium binding
KW - IgG
KW - Myeloma
UR - http://www.scopus.com/inward/record.url?scp=0021154077&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021154077&partnerID=8YFLogxK
U2 - 10.1007/BF00914965
DO - 10.1007/BF00914965
M3 - Article
C2 - 6736208
AN - SCOPUS:0021154077
VL - 4
SP - 185
EP - 196
JO - Journal of Clinical Immunology
JF - Journal of Clinical Immunology
SN - 0271-9142
IS - 3
ER -