A human synaptic vesicle monoamine transporter cDNA predicts posttranslational modifications, reveals chromosome 10 gene localization and identifies TaqI RFLPs

Christopher K. Surratt, Antonio M. Persico, Xiao Dong Yang, Stephanie R. Edgar, Geoffrey S. Bird, Anita L. Hawkins, Constance A. Griffin, Xiang Li, Ethylin W. Jabs, George R. Uhl

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat sequence, but displays one less consensus site for asparagine N-linked glycosylation and one more consensus site for phosphorylation by protein kinase C. The human SVMT gene maps to chromosome 10q25 using Southern blotting analysis of human/rodent hybrid cell lines and fluorescent in situ hybridization approaches. The cDNA, and a subclone, recognize TaqI polymorphisms that may prove useful to assess this gene's involvement in neuropsychiatric disorders involving monoaminergic brain systems.

Original languageEnglish
Pages (from-to)325-330
Number of pages6
JournalFEBS Letters
Volume318
Issue number3
DOIs
Publication statusPublished - Mar 8 1993

Fingerprint

Chromosomes, Human, Pair 10
Synaptic Vesicles
Post Translational Protein Processing
Chromosomes
Restriction Fragment Length Polymorphisms
Rats
Brain
Complementary DNA
Genes
Vesicular Monoamine Transport Proteins
Glycosylation
Phosphorylation
Asparagine
Polymorphism
Protein Kinase C
Screening
Cells
Amino Acids
Hybrid Cells
Southern Blotting

Keywords

  • Chromosomal mapping
  • Monoamine transporter
  • N-linked glycosylation
  • Polymorphism

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A human synaptic vesicle monoamine transporter cDNA predicts posttranslational modifications, reveals chromosome 10 gene localization and identifies TaqI RFLPs. / Surratt, Christopher K.; Persico, Antonio M.; Yang, Xiao Dong; Edgar, Stephanie R.; Bird, Geoffrey S.; Hawkins, Anita L.; Griffin, Constance A.; Li, Xiang; Jabs, Ethylin W.; Uhl, George R.

In: FEBS Letters, Vol. 318, No. 3, 08.03.1993, p. 325-330.

Research output: Contribution to journalArticle

Surratt, CK, Persico, AM, Yang, XD, Edgar, SR, Bird, GS, Hawkins, AL, Griffin, CA, Li, X, Jabs, EW & Uhl, GR 1993, 'A human synaptic vesicle monoamine transporter cDNA predicts posttranslational modifications, reveals chromosome 10 gene localization and identifies TaqI RFLPs', FEBS Letters, vol. 318, no. 3, pp. 325-330. https://doi.org/10.1016/0014-5793(93)80539-7
Surratt, Christopher K. ; Persico, Antonio M. ; Yang, Xiao Dong ; Edgar, Stephanie R. ; Bird, Geoffrey S. ; Hawkins, Anita L. ; Griffin, Constance A. ; Li, Xiang ; Jabs, Ethylin W. ; Uhl, George R. / A human synaptic vesicle monoamine transporter cDNA predicts posttranslational modifications, reveals chromosome 10 gene localization and identifies TaqI RFLPs. In: FEBS Letters. 1993 ; Vol. 318, No. 3. pp. 325-330.
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