A JC Virus-Induced Signal Is Required for Infection of Glial Cells by a Clathrin- and eps15-Dependent Pathway

W. Querbes, A. Benmerah, D. Tosoni, P. P. Di Fiore, Walter J. Atwood

Research output: Contribution to journalArticle

Abstract

Infectious entry of JC virus (JCV) into human glial cells occurs by receptor-mediated clathrin-dependent endocytosis. In this report we demonstrate that the tyrosine kinase inhibitor genistein blocks virus entry and inhibits infection. Transient expression of dominant-negative eps15 mutants, including a phosphorylation-defective mutant, inhibited both virus entry and infection. We also show that the JCV-induced signal activates the mitogen-activated protein kinases ERK1 and ERK2. These data demonstrate that JC virus binding to human glial cells induces an intracellular signal that is critical for entry and infection by a ligand-inducible clathrin-dependent mechanism.

Original languageEnglish
Pages (from-to)250-256
Number of pages7
JournalJournal of Virology
Volume78
Issue number1
DOIs
Publication statusPublished - Jan 2004

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ASJC Scopus subject areas

  • Immunology

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