A kinetic study on pantetheinase inhibition by disulfides

G. Pitari, G. Maurizi, P. Ascenzi, G. Ricci, S. Dupre

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The mammalian enzyme pantetheinase, which hydrolyzes pantetheine to pantothenic acid and cysteamine, is inhibited by many thiol reagents and activated by thiols. Two thiol groups of different reactivity and accessibility are involved in the catalytic process [Ricci, G., Nardini, M., Chiaraluce, R., Dupre, S. and Cavallini, D. (1986) Biochim. Biophys. Acta 870, 82-91]. The inhibition kinetics by some natural and synthetic disulfides [pantethine, cystamine, 5,5'-dithiobis(2-nitrobenzoic acid), 4,4'-dithiodipyridine and oxidized mercaptoethanol] has been studied by two experimental approaches, either by monitoring activity after incubation of the enzyme with the inhibitor or by determining the progress curves in the presence of substrate and inhibitor. Data reported here indicate that pantetheinase reacts irreversibly with various disulfides in a time-dependent manner with the formation of a mixed disulfide apparently preceeded by a conformational change, giving a modified E* form with new kinetic parameters. This modified form may be further competitively inhibited by disulfides interacting with the enzyme at the active site.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalEuropean Journal of Biochemistry
Volume226
Issue number1
DOIs
Publication statusPublished - 1994

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Disulfides
Kinetics
Sulfhydryl Compounds
Pantetheine
Enzymes
Cystamine
Pantothenic Acid
Dithionitrobenzoic Acid
Cysteamine
Sulfhydryl Reagents
Mercaptoethanol
Enzyme Inhibitors
Kinetic parameters
Catalytic Domain
pantetheinase
Monitoring
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

A kinetic study on pantetheinase inhibition by disulfides. / Pitari, G.; Maurizi, G.; Ascenzi, P.; Ricci, G.; Dupre, S.

In: European Journal of Biochemistry, Vol. 226, No. 1, 1994, p. 81-86.

Research output: Contribution to journalArticle

Pitari, G, Maurizi, G, Ascenzi, P, Ricci, G & Dupre, S 1994, 'A kinetic study on pantetheinase inhibition by disulfides', European Journal of Biochemistry, vol. 226, no. 1, pp. 81-86. https://doi.org/10.1111/j.1432-1033.1994.tb20028.x
Pitari, G. ; Maurizi, G. ; Ascenzi, P. ; Ricci, G. ; Dupre, S. / A kinetic study on pantetheinase inhibition by disulfides. In: European Journal of Biochemistry. 1994 ; Vol. 226, No. 1. pp. 81-86.
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