A large conductance Cl- channel revealed by patch-recordings in human fibroblasts

M. Nobile, L. J V Galietta

Research output: Contribution to journalArticlepeer-review

Abstract

A Cl- channel with large single-unit conductance and characteristic voltage-dependent inactivation was studied on cultured human fibroblasts. The channel was activated only after excision and lasting depolarization of the membrane patch. In inside-out configuration and in symmetrical 135 mM NaCl, the conductance was 300 pS. The channel was usually open at the membrane potentials between -20 to +20 mV, while more negative or positive voltages closed the channel. The time course of this apparent inactivation process was dependent on increasing potential. Recovery from inactivation was made possible by returning the membrane potential to 0 mV. The channel was selective to Cl- over Na+ with a PCl PNa of 6. The order of permeability among anions was: I>BrCl>isethionate>F>glutamate. The channel was blocked by internal application of a derivative of the diphenylamine-2-carboxilate (Blocker 144) but not by 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid.

Original languageEnglish
Pages (from-to)719-726
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume154
Issue number2
DOIs
Publication statusPublished - Jul 29 1988

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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