A lipemia-independent NanoDrop®-based score to identify hemolysis in plasma and serum samples

Valentina Appierto, Maurizio Callari, Elena Cavadini, Daniele Morelli, Maria Grazia Daidone, Paola Tiberio

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The identification and management of hemolyzed samples are crucial issues in the development of new blood-based biomarkers. Results: Using experiments of controlled hemolysis and lipemia and two plasma series from cancer patients, we developed and validated a lipemia-independent hemolysis score (HS). HS resulted strictly associated with the amount of lysed erythrocytes and with serum index measurement (reference method), highly reproducible, and able to identify as hemolyzed plasma/serum samples containing ≥6.1 mg/dl of free hemoglobin. Conclusion: We developed a simple, robust, sensitive, cost-effective, spectrophotometrically-based system to identify hemolyzed plasma/serum specimens. The procedure requires only 2 μl of sample, thus representing a useful tool for research studies and an essential pre-analytical quality control for an optimal biobanking of liquid biopsies.

Original languageEnglish
Pages (from-to)1215-1226
Number of pages12
JournalBioanalysis
Volume6
Issue number9
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Medical Laboratory Technology
  • Analytical Chemistry
  • Medicine(all)

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