A liver colony assay for a new hepatocyte phenotype as a step towards purifying new cellular phenotypes that arise during hepatocarcinogenesis

B. A. Laishes, L. Fink, B. I. Carr

Research output: Contribution to journalArticlepeer-review

Abstract

These experiments revealed that primary cell cultures derived from nodules of rats liver undergoing chemical carcinogenesis contained a larger amount of chemical resistant cells than did cultures derived from the liver of normal rats. Thus increasing the N-acetoxy-HAF (acetylaminofluorene) or N-acetoxy-HAF concentrations 100-fold from 10-6M to 10-4 resulted in an approximately five-fold drop in the percentage of surviving nodular liver derived cells, whereas this does not happen for normal liver cells. The nodular liver cells are also highly resistant to methotrexate, adriamycin and cycloheximide. C. Sirtori, Milan, Italy

Original languageEnglish
Pages (from-to)373-382
Number of pages10
JournalAnnals of the New York Academy of Sciences
VolumeVol.349
Publication statusPublished - 1980

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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