A macroscopic mathematical model for cell migration assays using a real-time cell analysis

Ezio Di Costanzo, Vincenzo Ingangi, Claudia Angelini, Maria Francesca Carfora, Maria Vincenza Carriero, Roberto Natalini

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Experiments of cell migration and chemotaxis assays have been classically performed in the so-called Boyden Chambers. A recent technology, xCELLigence Real Time Cell Analysis, is now allowing to monitor the cell migration in real time. This technology measures impedance changes caused by the gradual increase of electrode surface occupation by cells during the course of time and provide a Cell Index which is proportional to cellular morphology, spreading, ruffling and adhesion quality as well as cell number. In this paper we propose a macroscopic mathematical model, based on advection-reaction-diffusion partial differential equations, describing the cell migration assay using the real-time technology. We carried out numerical simulations to compare simulated model dynamics with data of observed biological experiments on three different cell lines and in two experimental settings: absence of chemotactic signals (basal migration) and presence of a chemoattractant. Overall we conclude that our minimal mathematical model is able to describe the phenomenon in the real time scale and numerical results show a good agreement with the experimental evidences.

Original languageEnglish
Article numbere0162553
JournalPLoS One
Volume11
Issue number9
DOIs
Publication statusPublished - Sep 1 2016

Fingerprint

Cell Migration Assays
cell movement
Assays
Theoretical Models
mathematical models
Mathematical models
assays
Technology
Chemotactic Factors
Advection
cells
Partial differential equations
chemoattractants
Dynamic models
chemotaxis
impedance
Adhesion
Experiments
Cells
dynamic models

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Di Costanzo, E., Ingangi, V., Angelini, C., Carfora, M. F., Carriero, M. V., & Natalini, R. (2016). A macroscopic mathematical model for cell migration assays using a real-time cell analysis. PLoS One, 11(9), [e0162553]. https://doi.org/10.1371/journal.pone.0162553

A macroscopic mathematical model for cell migration assays using a real-time cell analysis. / Di Costanzo, Ezio; Ingangi, Vincenzo; Angelini, Claudia; Carfora, Maria Francesca; Carriero, Maria Vincenza; Natalini, Roberto.

In: PLoS One, Vol. 11, No. 9, e0162553, 01.09.2016.

Research output: Contribution to journalArticle

Di Costanzo, E, Ingangi, V, Angelini, C, Carfora, MF, Carriero, MV & Natalini, R 2016, 'A macroscopic mathematical model for cell migration assays using a real-time cell analysis', PLoS One, vol. 11, no. 9, e0162553. https://doi.org/10.1371/journal.pone.0162553
Di Costanzo, Ezio ; Ingangi, Vincenzo ; Angelini, Claudia ; Carfora, Maria Francesca ; Carriero, Maria Vincenza ; Natalini, Roberto. / A macroscopic mathematical model for cell migration assays using a real-time cell analysis. In: PLoS One. 2016 ; Vol. 11, No. 9.
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