A mechanism for coupling exit from mitosis to partitioning of the nucleus

Allison J. Bardin, Rosella Visintin, Angelika Amon

Research output: Contribution to journalArticle

244 Citations (Scopus)

Abstract

Exit from mitosis must not occur prior to partitioning of chromosomes between daughter cells. We find that the GTP binding protein Tem1, a regulator of mitotic exit, is present on the spindle pole body that migrates into the bud during S phase and mitosis. Tem1's exchange factor, Lte1, localizes to the bud. Thus, Tem1 and Lte1 are present in the same cellular compartment (the bud) only after the nucleus enters the bud during nuclear division. We also find that the presence of Tem1 and Lte1 in the bud is required for mitotic exit. Our results suggest that the spatial segregation of Tem1 and Lte1 ensures that exit from mitosis only occurs after the genetic material is partitioned between mother and daughter cell.

Original languageEnglish
Pages (from-to)21-31
Number of pages11
JournalCell
Volume102
Issue number1
Publication statusPublished - Jul 7 2000

Fingerprint

Chromosomes
GTP-Binding Proteins
Mitosis
Poles
Spindle Pole Bodies
Cell Nucleus Division
S Phase
Stem Cells
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

A mechanism for coupling exit from mitosis to partitioning of the nucleus. / Bardin, Allison J.; Visintin, Rosella; Amon, Angelika.

In: Cell, Vol. 102, No. 1, 07.07.2000, p. 21-31.

Research output: Contribution to journalArticle

Bardin, AJ, Visintin, R & Amon, A 2000, 'A mechanism for coupling exit from mitosis to partitioning of the nucleus', Cell, vol. 102, no. 1, pp. 21-31.
Bardin, Allison J. ; Visintin, Rosella ; Amon, Angelika. / A mechanism for coupling exit from mitosis to partitioning of the nucleus. In: Cell. 2000 ; Vol. 102, No. 1. pp. 21-31.
@article{875084faa28242428639e1756fed20f1,
title = "A mechanism for coupling exit from mitosis to partitioning of the nucleus",
abstract = "Exit from mitosis must not occur prior to partitioning of chromosomes between daughter cells. We find that the GTP binding protein Tem1, a regulator of mitotic exit, is present on the spindle pole body that migrates into the bud during S phase and mitosis. Tem1's exchange factor, Lte1, localizes to the bud. Thus, Tem1 and Lte1 are present in the same cellular compartment (the bud) only after the nucleus enters the bud during nuclear division. We also find that the presence of Tem1 and Lte1 in the bud is required for mitotic exit. Our results suggest that the spatial segregation of Tem1 and Lte1 ensures that exit from mitosis only occurs after the genetic material is partitioned between mother and daughter cell.",
author = "Bardin, {Allison J.} and Rosella Visintin and Angelika Amon",
year = "2000",
month = "7",
day = "7",
language = "English",
volume = "102",
pages = "21--31",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - A mechanism for coupling exit from mitosis to partitioning of the nucleus

AU - Bardin, Allison J.

AU - Visintin, Rosella

AU - Amon, Angelika

PY - 2000/7/7

Y1 - 2000/7/7

N2 - Exit from mitosis must not occur prior to partitioning of chromosomes between daughter cells. We find that the GTP binding protein Tem1, a regulator of mitotic exit, is present on the spindle pole body that migrates into the bud during S phase and mitosis. Tem1's exchange factor, Lte1, localizes to the bud. Thus, Tem1 and Lte1 are present in the same cellular compartment (the bud) only after the nucleus enters the bud during nuclear division. We also find that the presence of Tem1 and Lte1 in the bud is required for mitotic exit. Our results suggest that the spatial segregation of Tem1 and Lte1 ensures that exit from mitosis only occurs after the genetic material is partitioned between mother and daughter cell.

AB - Exit from mitosis must not occur prior to partitioning of chromosomes between daughter cells. We find that the GTP binding protein Tem1, a regulator of mitotic exit, is present on the spindle pole body that migrates into the bud during S phase and mitosis. Tem1's exchange factor, Lte1, localizes to the bud. Thus, Tem1 and Lte1 are present in the same cellular compartment (the bud) only after the nucleus enters the bud during nuclear division. We also find that the presence of Tem1 and Lte1 in the bud is required for mitotic exit. Our results suggest that the spatial segregation of Tem1 and Lte1 ensures that exit from mitosis only occurs after the genetic material is partitioned between mother and daughter cell.

UR - http://www.scopus.com/inward/record.url?scp=0034616929&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034616929&partnerID=8YFLogxK

M3 - Article

C2 - 10929710

AN - SCOPUS:0034616929

VL - 102

SP - 21

EP - 31

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1

ER -