A mechanism for inhibiting the SUMO pathway

Roberto Boggio, Riccardo Colombo, Ronald T. Hay, Giulio F. Draetta, Susanna Chiocca

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

The SUMO pathway parallels the classical ubiquitinylation pathway with three discrete steps: activation involving the enzyme E1, conjugation involving the E2 enzyme UBC9, and substrate modification through the cooperative association of UBC9 and E3 ligases. We report here that the adenoviral protein Gam1 inhibits the SUMO pathway by interfering with the activity of E1 (SAE1/SAE2). In vivo, Gam1 expression leads to SAE1/SAE2 inactivation, both SAE1/SAE2 and UBC9 disappearance, and overall inhibition of protein sumoylation. This results in transcriptional activation of some promoters and is directly linked to inhibition of sumoylation of the transcriptional activators involved. Our results identify a mechanism for interfering with the SUMO pathway and with transcription that could have an impact in the design of novel pharmaceutical agents. They also point out once again to the extraordinary ability of eukaryotic viruses to interfere with the biology of host cells by targeting fundamental biochemical processes.

Original languageEnglish
Pages (from-to)549-561
Number of pages13
JournalMolecular Cell
Volume16
Issue number4
DOIs
Publication statusPublished - Nov 19 2004

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Sumoylation
Biochemical Phenomena
Enzyme Activation
Ubiquitin-Protein Ligases
Transcriptional Activation
Cell Biology
Proteins
Viruses
Enzymes
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Boggio, R., Colombo, R., Hay, R. T., Draetta, G. F., & Chiocca, S. (2004). A mechanism for inhibiting the SUMO pathway. Molecular Cell, 16(4), 549-561. https://doi.org/10.1016/j.molcel.2004.11.007

A mechanism for inhibiting the SUMO pathway. / Boggio, Roberto; Colombo, Riccardo; Hay, Ronald T.; Draetta, Giulio F.; Chiocca, Susanna.

In: Molecular Cell, Vol. 16, No. 4, 19.11.2004, p. 549-561.

Research output: Contribution to journalArticle

Boggio, R, Colombo, R, Hay, RT, Draetta, GF & Chiocca, S 2004, 'A mechanism for inhibiting the SUMO pathway', Molecular Cell, vol. 16, no. 4, pp. 549-561. https://doi.org/10.1016/j.molcel.2004.11.007
Boggio, Roberto ; Colombo, Riccardo ; Hay, Ronald T. ; Draetta, Giulio F. ; Chiocca, Susanna. / A mechanism for inhibiting the SUMO pathway. In: Molecular Cell. 2004 ; Vol. 16, No. 4. pp. 549-561.
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