A melanoma immune response signature including Human Leukocyte Antigen-E

Elisa Tremante; Agnese Ginebri; Elisa Lo Monaco; Barbara Benassi; Pasquale Frascione; Paola Grammatico; Sandra Cappellacci; Caterina Catricalà; Diego Arcelli; Pier Giorgio Natali; Franco Di Filippo; Marcella Mottolese; Paolo Visca; Maria Benevolo; Patrizio Giacomini

doi:10.1111/pcmr.12164

A melanoma immune response signature including Human Leukocyte Antigen-E

Elisa Tremante, Agnese Ginebri, Elisa Lo Monaco, Barbara Benassi, Pasquale Frascione, Paola Grammatico, Sandra Cappellacci, Caterina Catricalà, Diego Arcelli, Pier Giorgio Natali, Franco Di Filippo, Marcella Mottolese, Paolo Visca, Maria Benevolo, Patrizio Giacomini

Research output: Contribution to journal › Article › peer-review

Abstract

Paired cultures of early-passage melanoma cells and melanocytes were established from metastatic lesions and the uninvolved skin of five patients. In this stringent autologous setting, cDNA profiling was used to analyze a subset of 1477 genes selected by the Gene Ontology term 'immune response'. Human Leukocyte Antigen E (HLA-E) was ranked 19th among melanoma-overexpressed genes and was embedded in a transformation signature including its preferred peptide ligand donors HLA-A, HLA-B, HLA-C, and HLA-G. Mostly undetectable in normal skin and 39 nevi (including rare and atypical lesions), HLA-E was detected by immunohistochemistry in 17/30 (57%) and 32/48 (67%) primary and metastatic lesions, respectively. Accordingly, surface HLA-E was higher on melanoma cells than on melanocytes and protected the former (6/6 cell lines) from lysis by natural killer (NK) cells, functionally counteracting co-expressed triggering ligands. Although lacking HLA-E, melanocytes (4/4 cultures) were nevertheless (and surprisingly) fully protected from NK cell lysis.

Original language	English
Pages (from-to)	103-112
Number of pages	10
Journal	Pigment Cell and Melanoma Research
Volume	27
Issue number	1
DOIs	https://doi.org/10.1111/pcmr.12164
Publication status	Published - Jan 2014

Keywords

cDNA arrays
HLA-E
Melanocytes
Melanoma
NK cells

ASJC Scopus subject areas

Dermatology
Oncology
Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1111/pcmr.12164

Cite this

Tremante, E., Ginebri, A., Lo Monaco, E., Benassi, B., Frascione, P., Grammatico, P., Cappellacci, S., Catricalà, C., Arcelli, D., Natali, P. G., Di Filippo, F., Mottolese, M., Visca, P., Benevolo, M., & Giacomini, P. (2014). A melanoma immune response signature including Human Leukocyte Antigen-E. Pigment Cell and Melanoma Research, 27(1), 103-112. https://doi.org/10.1111/pcmr.12164

Tremante, E, Ginebri, A, Lo Monaco, E, Benassi, B, Frascione, P, Grammatico, P, Cappellacci, S, Catricalà, C, Arcelli, D, Natali, PG, Di Filippo, F, Mottolese, M, Visca, P , Benevolo, M & Giacomini, P 2014, 'A melanoma immune response signature including Human Leukocyte Antigen-E', Pigment Cell and Melanoma Research, vol. 27, no. 1, pp. 103-112. https://doi.org/10.1111/pcmr.12164

@article{e8c5ea8195fa4635a6be1c6dc68ca465,

title = "A melanoma immune response signature including Human Leukocyte Antigen-E",

abstract = "Paired cultures of early-passage melanoma cells and melanocytes were established from metastatic lesions and the uninvolved skin of five patients. In this stringent autologous setting, cDNA profiling was used to analyze a subset of 1477 genes selected by the Gene Ontology term 'immune response'. Human Leukocyte Antigen E (HLA-E) was ranked 19th among melanoma-overexpressed genes and was embedded in a transformation signature including its preferred peptide ligand donors HLA-A, HLA-B, HLA-C, and HLA-G. Mostly undetectable in normal skin and 39 nevi (including rare and atypical lesions), HLA-E was detected by immunohistochemistry in 17/30 (57%) and 32/48 (67%) primary and metastatic lesions, respectively. Accordingly, surface HLA-E was higher on melanoma cells than on melanocytes and protected the former (6/6 cell lines) from lysis by natural killer (NK) cells, functionally counteracting co-expressed triggering ligands. Although lacking HLA-E, melanocytes (4/4 cultures) were nevertheless (and surprisingly) fully protected from NK cell lysis.",

keywords = "cDNA arrays, HLA-E, Melanocytes, Melanoma, NK cells",

author = "Elisa Tremante and Agnese Ginebri and {Lo Monaco}, Elisa and Barbara Benassi and Pasquale Frascione and Paola Grammatico and Sandra Cappellacci and Caterina Catrical{\`a} and Diego Arcelli and Natali, {Pier Giorgio} and {Di Filippo}, Franco and Marcella Mottolese and Paolo Visca and Maria Benevolo and Patrizio Giacomini",

year = "2014",

month = jan,

doi = "10.1111/pcmr.12164",

language = "English",

volume = "27",

pages = "103--112",

journal = "Pigment Cell and Melanoma Research",

issn = "1755-1471",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - A melanoma immune response signature including Human Leukocyte Antigen-E

AU - Tremante, Elisa

AU - Ginebri, Agnese

AU - Lo Monaco, Elisa

AU - Benassi, Barbara

AU - Frascione, Pasquale

AU - Grammatico, Paola

AU - Cappellacci, Sandra

AU - Catricalà, Caterina

AU - Arcelli, Diego

AU - Natali, Pier Giorgio

AU - Di Filippo, Franco

AU - Mottolese, Marcella

AU - Visca, Paolo

AU - Benevolo, Maria

AU - Giacomini, Patrizio

PY - 2014/1

Y1 - 2014/1

N2 - Paired cultures of early-passage melanoma cells and melanocytes were established from metastatic lesions and the uninvolved skin of five patients. In this stringent autologous setting, cDNA profiling was used to analyze a subset of 1477 genes selected by the Gene Ontology term 'immune response'. Human Leukocyte Antigen E (HLA-E) was ranked 19th among melanoma-overexpressed genes and was embedded in a transformation signature including its preferred peptide ligand donors HLA-A, HLA-B, HLA-C, and HLA-G. Mostly undetectable in normal skin and 39 nevi (including rare and atypical lesions), HLA-E was detected by immunohistochemistry in 17/30 (57%) and 32/48 (67%) primary and metastatic lesions, respectively. Accordingly, surface HLA-E was higher on melanoma cells than on melanocytes and protected the former (6/6 cell lines) from lysis by natural killer (NK) cells, functionally counteracting co-expressed triggering ligands. Although lacking HLA-E, melanocytes (4/4 cultures) were nevertheless (and surprisingly) fully protected from NK cell lysis.

AB - Paired cultures of early-passage melanoma cells and melanocytes were established from metastatic lesions and the uninvolved skin of five patients. In this stringent autologous setting, cDNA profiling was used to analyze a subset of 1477 genes selected by the Gene Ontology term 'immune response'. Human Leukocyte Antigen E (HLA-E) was ranked 19th among melanoma-overexpressed genes and was embedded in a transformation signature including its preferred peptide ligand donors HLA-A, HLA-B, HLA-C, and HLA-G. Mostly undetectable in normal skin and 39 nevi (including rare and atypical lesions), HLA-E was detected by immunohistochemistry in 17/30 (57%) and 32/48 (67%) primary and metastatic lesions, respectively. Accordingly, surface HLA-E was higher on melanoma cells than on melanocytes and protected the former (6/6 cell lines) from lysis by natural killer (NK) cells, functionally counteracting co-expressed triggering ligands. Although lacking HLA-E, melanocytes (4/4 cultures) were nevertheless (and surprisingly) fully protected from NK cell lysis.

KW - cDNA arrays

KW - HLA-E

KW - Melanocytes

KW - Melanoma

KW - NK cells

UR - http://www.scopus.com/inward/record.url?scp=84890788906&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84890788906&partnerID=8YFLogxK

U2 - 10.1111/pcmr.12164

DO - 10.1111/pcmr.12164

M3 - Article

C2 - 24011128

AN - SCOPUS:84890788906

VL - 27

SP - 103

EP - 112

JO - Pigment Cell and Melanoma Research

JF - Pigment Cell and Melanoma Research

SN - 1755-1471

IS - 1

ER -

A melanoma immune response signature including Human Leukocyte Antigen-E

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this