Abstract
The unit describes protocols for isolating and characterizing extracellular vesicles (EVs) derived from human adipose tissue-derived mesenchymal stromal cells (MSCs). EVs are a mixed population of membrane-surrounded structures with overlapping composition and size. Advances made in recent years have led to a better understanding of the biological role of EVs. In particular, they can be considered key factors responsible for MSC-paracrine activity, mediating their anti-inflammatory effects towards innate immune cells, such as macrophages. The topics comprise description of the MSC-conditioned medium containing vesicles preparation, EV isolation, and characterization mainly by specifically set up flow cytometry and electron microscopy approaches, and in vitro methodologies involved in testing the EV anti-inflammatory capacity. The procedures described here can be easily reproduced and can be employed regardless of the type of progenitor cells used to secrete EVs.
| Original language | English |
|---|---|
| Article number | e55 |
| Journal | Current Protocols in Stem Cell Biology |
| Volume | 46 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Aug 1 2018 |
| Externally published | Yes |
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Keywords
- extracellular vesicles
- inflammation
- macrophages
- mesenchymal stromal cells
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
A Method for Isolating and Characterizing Mesenchymal Stromal Cell-derived Extracellular Vesicles. / Lo Sicco, Claudia; Reverberi, Daniele; Pascucci, Luisa; Tasso, Roberta.
In: Current Protocols in Stem Cell Biology, Vol. 46, No. 1, e55, 01.08.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A Method for Isolating and Characterizing Mesenchymal Stromal Cell-derived Extracellular Vesicles
AU - Lo Sicco, Claudia
AU - Reverberi, Daniele
AU - Pascucci, Luisa
AU - Tasso, Roberta
PY - 2018/8/1
Y1 - 2018/8/1
N2 - The unit describes protocols for isolating and characterizing extracellular vesicles (EVs) derived from human adipose tissue-derived mesenchymal stromal cells (MSCs). EVs are a mixed population of membrane-surrounded structures with overlapping composition and size. Advances made in recent years have led to a better understanding of the biological role of EVs. In particular, they can be considered key factors responsible for MSC-paracrine activity, mediating their anti-inflammatory effects towards innate immune cells, such as macrophages. The topics comprise description of the MSC-conditioned medium containing vesicles preparation, EV isolation, and characterization mainly by specifically set up flow cytometry and electron microscopy approaches, and in vitro methodologies involved in testing the EV anti-inflammatory capacity. The procedures described here can be easily reproduced and can be employed regardless of the type of progenitor cells used to secrete EVs.
AB - The unit describes protocols for isolating and characterizing extracellular vesicles (EVs) derived from human adipose tissue-derived mesenchymal stromal cells (MSCs). EVs are a mixed population of membrane-surrounded structures with overlapping composition and size. Advances made in recent years have led to a better understanding of the biological role of EVs. In particular, they can be considered key factors responsible for MSC-paracrine activity, mediating their anti-inflammatory effects towards innate immune cells, such as macrophages. The topics comprise description of the MSC-conditioned medium containing vesicles preparation, EV isolation, and characterization mainly by specifically set up flow cytometry and electron microscopy approaches, and in vitro methodologies involved in testing the EV anti-inflammatory capacity. The procedures described here can be easily reproduced and can be employed regardless of the type of progenitor cells used to secrete EVs.
KW - extracellular vesicles
KW - inflammation
KW - macrophages
KW - mesenchymal stromal cells
UR - http://www.scopus.com/inward/record.url?scp=85050964140&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85050964140&partnerID=8YFLogxK
U2 - 10.1002/cpsc.55
DO - 10.1002/cpsc.55
M3 - Article
AN - SCOPUS:85050964140
VL - 46
JO - American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics
JF - American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics
SN - 1552-4841
IS - 1
M1 - e55
ER -