A method for the determination of the adherence of granulocytes to microtitre plates

G. F. Santini, S. Battistin, P. De Paoli, D. Villalta, M. Borean, G. Basaglia

Research output: Contribution to journalArticle

Abstract

We report a new partially automated method for the measurement of the adherence of PMN in vitro. Adherence to a plastic surface was detected by measuring leukocyte alkaline phosphatase activity of the adherent cells, with a Titertek Multiscan system. Using three different cellular concentrations (1 × 106, 5 × 105, 2.5 × 105 PMN/ml) the response curve was linear to 45 min and adhesion was maximal by 30 min. The specificity of the reaction was acceptable as was the assay reproducibility (intra-assay coefficient of variation

Original languageEnglish
Pages (from-to)153-156
Number of pages4
JournalJournal of Immunological Methods
Volume100
Issue number1-2
DOIs
Publication statusPublished - Jun 26 1987

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Keywords

  • Adherence
  • Alkaline phosphatase
  • ELISA plate
  • Polymorphonuclear leukocyte

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Santini, G. F., Battistin, S., De Paoli, P., Villalta, D., Borean, M., & Basaglia, G. (1987). A method for the determination of the adherence of granulocytes to microtitre plates. Journal of Immunological Methods, 100(1-2), 153-156. https://doi.org/10.1016/0022-1759(87)90184-0