A method for the determination of the adherence of granulocytes to microtitre plates

G. F. Santini; S. Battistin; P. De Paoli; D. Villalta; M. Borean; G. Basaglia

doi:10.1016/0022-1759(87)90184-0

A method for the determination of the adherence of granulocytes to microtitre plates

G. F. Santini, S. Battistin, P. De Paoli, D. Villalta, M. Borean, G. Basaglia

Centro di Riferimento Oncologico

Research output: Contribution to journal › Article

Abstract

We report a new partially automated method for the measurement of the adherence of PMN in vitro. Adherence to a plastic surface was detected by measuring leukocyte alkaline phosphatase activity of the adherent cells, with a Titertek Multiscan system. Using three different cellular concentrations (1 × 10⁶, 5 × 10⁵, 2.5 × 10⁵ PMN/ml) the response curve was linear to 45 min and adhesion was maximal by 30 min. The specificity of the reaction was acceptable as was the assay reproducibility (intra-assay coefficient of variation

Original language	English
Pages (from-to)	153-156
Number of pages	4
Journal	Journal of Immunological Methods
Volume	100
Issue number	1-2
DOIs	https://doi.org/10.1016/0022-1759(87)90184-0
Publication status	Published - Jun 26 1987

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Keywords

Adherence
Alkaline phosphatase
ELISA plate
Polymorphonuclear leukocyte

ASJC Scopus subject areas

Biotechnology
Immunology

Cite this

Santini, G. F., Battistin, S., De Paoli, P., Villalta, D., Borean, M., & Basaglia, G. (1987). A method for the determination of the adherence of granulocytes to microtitre plates. Journal of Immunological Methods, 100(1-2), 153-156. https://doi.org/10.1016/0022-1759(87)90184-0

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AU - Borean, M.

AU - Basaglia, G.

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KW - ELISA plate

KW - Polymorphonuclear leukocyte

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10.1016/0022-1759(87)90184-0