TY - JOUR
T1 - A method for the determination of the adherence of granulocytes to microtitre plates
AU - Santini, G. F.
AU - Battistin, S.
AU - De Paoli, P.
AU - Villalta, D.
AU - Borean, M.
AU - Basaglia, G.
PY - 1987/6/26
Y1 - 1987/6/26
N2 - We report a new partially automated method for the measurement of the adherence of PMN in vitro. Adherence to a plastic surface was detected by measuring leukocyte alkaline phosphatase activity of the adherent cells, with a Titertek Multiscan system. Using three different cellular concentrations (1 × 106, 5 × 105, 2.5 × 105 PMN/ml) the response curve was linear to 45 min and adhesion was maximal by 30 min. The specificity of the reaction was acceptable as was the assay reproducibility (intra-assay coefficient of variation
AB - We report a new partially automated method for the measurement of the adherence of PMN in vitro. Adherence to a plastic surface was detected by measuring leukocyte alkaline phosphatase activity of the adherent cells, with a Titertek Multiscan system. Using three different cellular concentrations (1 × 106, 5 × 105, 2.5 × 105 PMN/ml) the response curve was linear to 45 min and adhesion was maximal by 30 min. The specificity of the reaction was acceptable as was the assay reproducibility (intra-assay coefficient of variation
KW - Adherence
KW - Alkaline phosphatase
KW - ELISA plate
KW - Polymorphonuclear leukocyte
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U2 - 10.1016/0022-1759(87)90184-0
DO - 10.1016/0022-1759(87)90184-0
M3 - Article
C2 - 3598195
AN - SCOPUS:0023221808
VL - 100
SP - 153
EP - 156
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1-2
ER -