We present a simple HPLC assay for the determination of morphine, and morphine-6-glucuronide in plasma. Plasma is extracted on Sep-pak(R) cartridges, samples loaded on a C18 column and eluted in 5% acetonitrile adjusted to pH 3,7. The method detects 5,0 ng/mL of the opiate and its metabolites, and therefore is suitable for monitoring morphine administration during therapy.
|Number of pages||5|
|Journal||Giornale Italiano di Chimica Clinica|
|Publication status||Published - 1994|
ASJC Scopus subject areas
- Clinical Biochemistry