TY - JOUR
T1 - A method for the simultaneous determination of morphine and morphine-6-glucuronide in plasma by HPLC
AU - Breda, M.
AU - Bianchi, M.
AU - Ripamonti, C.
AU - Lucarelli, C.
AU - Panerai, A. E.
PY - 1994
Y1 - 1994
N2 - We present a simple HPLC assay for the determination of morphine, and morphine-6-glucuronide in plasma. Plasma is extracted on Sep-pak(R) cartridges, samples loaded on a C18 column and eluted in 5% acetonitrile adjusted to pH 3,7. The method detects 5,0 ng/mL of the opiate and its metabolites, and therefore is suitable for monitoring morphine administration during therapy.
AB - We present a simple HPLC assay for the determination of morphine, and morphine-6-glucuronide in plasma. Plasma is extracted on Sep-pak(R) cartridges, samples loaded on a C18 column and eluted in 5% acetonitrile adjusted to pH 3,7. The method detects 5,0 ng/mL of the opiate and its metabolites, and therefore is suitable for monitoring morphine administration during therapy.
KW - morphine
KW - morphine-3-glucuronide
KW - morphine-6-glucuronide
UR - http://www.scopus.com/inward/record.url?scp=0028662007&partnerID=8YFLogxK
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M3 - Article
AN - SCOPUS:0028662007
VL - 19
SP - 53
EP - 57
JO - Giornale Italiano di Chimica Clinica
JF - Giornale Italiano di Chimica Clinica
SN - 0392-2227
IS - 1
ER -