A method for the simultaneous determination of morphine and morphine-6-glucuronide in plasma by HPLC

M. Breda, M. Bianchi, C. Ripamonti, C. Lucarelli, A. E. Panerai

Research output: Contribution to journalArticlepeer-review

Abstract

We present a simple HPLC assay for the determination of morphine, and morphine-6-glucuronide in plasma. Plasma is extracted on Sep-pak(R) cartridges, samples loaded on a C18 column and eluted in 5% acetonitrile adjusted to pH 3,7. The method detects 5,0 ng/mL of the opiate and its metabolites, and therefore is suitable for monitoring morphine administration during therapy.

Original languageEnglish
Pages (from-to)53-57
Number of pages5
JournalGiornale Italiano di Chimica Clinica
Volume19
Issue number1
Publication statusPublished - 1994

Keywords

  • morphine
  • morphine-3-glucuronide
  • morphine-6-glucuronide

ASJC Scopus subject areas

  • Clinical Biochemistry

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