A micro/nanoscale surface mechanical study on morpho-functional changes in multilineage-differentiated human mesenchymal stem cells

Serena Danti; Mario D'Acunto; Luisa Trombi; Stefano Berrettini; Andrea Pietrabissa

doi:10.1002/mabi.200600271

A micro/nanoscale surface mechanical study on morpho-functional changes in multilineage-differentiated human mesenchymal stem cells

Serena Danti, Mario D'Acunto, Luisa Trombi, Stefano Berrettini, Andrea Pietrabissa

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

In recent years MSCs have become a very attractive tool in tissue engineering and regenerative medicine because of their ability to be committed along several lineages through chemical or physical stimuli. Nevertheless their therapeutic potential and plasticity are not yet totally understood. This report describes the use of AFM together with conventional microscopies to obtain mechanical information on cell surfaces and deposited extra cellular matrix molecules, after inducing the differentiation of human MSCs towards three typical mesoderm phenotypes. The aim is to correlate morphological, functional, and mechanical aspects of human MSCs to obtain a deeper understanding of their great potential. (Graph Presented).

Original language	English
Pages (from-to)	589-598
Number of pages	10
Journal	Macromolecular Bioscience
Volume	7
Issue number	5
DOIs	https://doi.org/10.1002/mabi.200600271
Publication status	Published - May 10 2007

Keywords

Atomic force microscopy (AFM)
Cell topography
Differentiation
Human mesenchymal stem cells (hMSC)
Imaging

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Biochemistry
Bioengineering
Biomaterials
Materials Chemistry
Polymers and Plastics

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abstract = "In recent years MSCs have become a very attractive tool in tissue engineering and regenerative medicine because of their ability to be committed along several lineages through chemical or physical stimuli. Nevertheless their therapeutic potential and plasticity are not yet totally understood. This report describes the use of AFM together with conventional microscopies to obtain mechanical information on cell surfaces and deposited extra cellular matrix molecules, after inducing the differentiation of human MSCs towards three typical mesoderm phenotypes. The aim is to correlate morphological, functional, and mechanical aspects of human MSCs to obtain a deeper understanding of their great potential. (Graph Presented).",

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AU - Berrettini, Stefano

AU - Pietrabissa, Andrea

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KW - Cell topography

KW - Differentiation

KW - Human mesenchymal stem cells (hMSC)

KW - Imaging

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