Abstract
Background: In a previous publication we described a method for Jk a/Jk b, Fy a/Fy b, S/s, K/k, Kp a/Kp b, Js a/Js b, Co a/Co b, and Lu a/Lu b genotyping based on a microsphere suspension array. Here, an improved version of the assay is presented. Methods: Two multiplex polymerase chain reactions (PCR) were developed: one for amplification of samples routinely tested and the other for those systems that are tested less frequently. Each biotinylated PCR product is hybridized in a single multiplex assay. A total of 2,020 samples were analyzed, and the genotypes were compared to the blood group phenotypes. Results: There have been no discrepancies with the serology results other than null and/or weak phenotypes. Conclusion: In its present form, the method presented here has the capacity to genotype hundreds of a samples in few hours with a high concordance rate with serology.
| Original language | English |
|---|---|
| Pages (from-to) | 336-338 |
| Number of pages | 3 |
| Journal | Transfusion Medicine and Hemotherapy |
| Volume | 37 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 2010 |
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Keywords
- Genotyping
- Red blood cell antigens
- Red blood cells
ASJC Scopus subject areas
- Hematology
- Immunology and Allergy
Cite this
A microsphere-based suspension array for blood group molecular typing : An update. / Drago, Francesca; Karpasitou, Katerina; Spinardi, Laura; Crespiatico, Loretta; Scalamogna, Mario; Poli, Francesca.
In: Transfusion Medicine and Hemotherapy, Vol. 37, No. 6, 12.2010, p. 336-338.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A microsphere-based suspension array for blood group molecular typing
T2 - An update
AU - Drago, Francesca
AU - Karpasitou, Katerina
AU - Spinardi, Laura
AU - Crespiatico, Loretta
AU - Scalamogna, Mario
AU - Poli, Francesca
PY - 2010/12
Y1 - 2010/12
N2 - Background: In a previous publication we described a method for Jk a/Jk b, Fy a/Fy b, S/s, K/k, Kp a/Kp b, Js a/Js b, Co a/Co b, and Lu a/Lu b genotyping based on a microsphere suspension array. Here, an improved version of the assay is presented. Methods: Two multiplex polymerase chain reactions (PCR) were developed: one for amplification of samples routinely tested and the other for those systems that are tested less frequently. Each biotinylated PCR product is hybridized in a single multiplex assay. A total of 2,020 samples were analyzed, and the genotypes were compared to the blood group phenotypes. Results: There have been no discrepancies with the serology results other than null and/or weak phenotypes. Conclusion: In its present form, the method presented here has the capacity to genotype hundreds of a samples in few hours with a high concordance rate with serology.
AB - Background: In a previous publication we described a method for Jk a/Jk b, Fy a/Fy b, S/s, K/k, Kp a/Kp b, Js a/Js b, Co a/Co b, and Lu a/Lu b genotyping based on a microsphere suspension array. Here, an improved version of the assay is presented. Methods: Two multiplex polymerase chain reactions (PCR) were developed: one for amplification of samples routinely tested and the other for those systems that are tested less frequently. Each biotinylated PCR product is hybridized in a single multiplex assay. A total of 2,020 samples were analyzed, and the genotypes were compared to the blood group phenotypes. Results: There have been no discrepancies with the serology results other than null and/or weak phenotypes. Conclusion: In its present form, the method presented here has the capacity to genotype hundreds of a samples in few hours with a high concordance rate with serology.
KW - Genotyping
KW - Red blood cell antigens
KW - Red blood cells
UR - http://www.scopus.com/inward/record.url?scp=78649711939&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78649711939&partnerID=8YFLogxK
U2 - 10.1159/000322039
DO - 10.1159/000322039
M3 - Article
AN - SCOPUS:78649711939
VL - 37
SP - 336
EP - 338
JO - Transfusion Medicine and Hemotherapy
JF - Transfusion Medicine and Hemotherapy
SN - 1660-3796
IS - 6
ER -