A microsphere-based suspension array for blood group molecular typing: An update

Francesca Drago, Katerina Karpasitou, Laura Spinardi, Loretta Crespiatico, Mario Scalamogna, Francesca Poli

Research output: Contribution to journalArticlepeer-review

Abstract

Background: In a previous publication we described a method for Jk a/Jk b, Fy a/Fy b, S/s, K/k, Kp a/Kp b, Js a/Js b, Co a/Co b, and Lu a/Lu b genotyping based on a microsphere suspension array. Here, an improved version of the assay is presented. Methods: Two multiplex polymerase chain reactions (PCR) were developed: one for amplification of samples routinely tested and the other for those systems that are tested less frequently. Each biotinylated PCR product is hybridized in a single multiplex assay. A total of 2,020 samples were analyzed, and the genotypes were compared to the blood group phenotypes. Results: There have been no discrepancies with the serology results other than null and/or weak phenotypes. Conclusion: In its present form, the method presented here has the capacity to genotype hundreds of a samples in few hours with a high concordance rate with serology.

Original languageEnglish
Pages (from-to)336-338
Number of pages3
JournalTransfusion Medicine and Hemotherapy
Volume37
Issue number6
DOIs
Publication statusPublished - Dec 2010

Keywords

  • Genotyping
  • Red blood cell antigens
  • Red blood cells

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy

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