TY - JOUR
T1 - A microsphere-based suspension array for blood group molecular typing
T2 - An update
AU - Drago, Francesca
AU - Karpasitou, Katerina
AU - Spinardi, Laura
AU - Crespiatico, Loretta
AU - Scalamogna, Mario
AU - Poli, Francesca
PY - 2010/12
Y1 - 2010/12
N2 - Background: In a previous publication we described a method for Jk
a/Jk
b, Fy
a/Fy
b, S/s, K/k, Kp
a/Kp
b, Js
a/Js
b, Co
a/Co
b, and Lu
a/Lu
b genotyping based on a microsphere suspension array. Here, an improved version of the assay is presented. Methods: Two multiplex polymerase chain reactions (PCR) were developed: one for amplification of samples routinely tested and the other for those systems that are tested less frequently. Each biotinylated PCR product is hybridized in a single multiplex assay. A total of 2,020 samples were analyzed, and the genotypes were compared to the blood group phenotypes. Results: There have been no discrepancies with the serology results other than null and/or weak phenotypes. Conclusion: In its present form, the method presented here has the capacity to genotype hundreds of a samples in few hours with a high concordance rate with serology.
AB - Background: In a previous publication we described a method for Jk
a/Jk
b, Fy
a/Fy
b, S/s, K/k, Kp
a/Kp
b, Js
a/Js
b, Co
a/Co
b, and Lu
a/Lu
b genotyping based on a microsphere suspension array. Here, an improved version of the assay is presented. Methods: Two multiplex polymerase chain reactions (PCR) were developed: one for amplification of samples routinely tested and the other for those systems that are tested less frequently. Each biotinylated PCR product is hybridized in a single multiplex assay. A total of 2,020 samples were analyzed, and the genotypes were compared to the blood group phenotypes. Results: There have been no discrepancies with the serology results other than null and/or weak phenotypes. Conclusion: In its present form, the method presented here has the capacity to genotype hundreds of a samples in few hours with a high concordance rate with serology.
KW - Genotyping
KW - Red blood cell antigens
KW - Red blood cells
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U2 - 10.1159/000322039
DO - 10.1159/000322039
M3 - Article
AN - SCOPUS:78649711939
VL - 37
SP - 336
EP - 338
JO - Infusionstherapie und Klinische Ernahrung - Forschung und Praxis
JF - Infusionstherapie und Klinische Ernahrung - Forschung und Praxis
SN - 1660-3796
IS - 6
ER -