A microsystem to evaluate the synthesis of [3H]leucine labeled proteins by macrophages

Luigi Varesio; Alessandra Eva

doi:10.1016/0022-1759(80)90210-0

A microsystem to evaluate the synthesis of [3H]leucine labeled proteins by macrophages

Luigi Varesio, Alessandra Eva

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article

Abstract

A method is described for evaluating protein synthesis by adherent MF{cyrillic} by measuring the incorporation of [³H]leucine into TCA precipitable material. By using guanidine-HCl it was possible to remove completely the radiolabeled proteins from the adherent cells that were cultured in microwells, and retain TCA precipitable material. This procedure enabled us to harvest the TCA precipitable proteins with semiautomatic cell harvester. The guanidine-HCl treatment did not affect the recovery of the radioactive proteins and did not alter the sensitivity of the assay. This method is very simple and rapid and, since it is suitable for processing microcultures, permits detailed studies on the biology of small numbers of MF{cyrillic}.

Original language	English
Pages (from-to)	231-238
Number of pages	8
Journal	Journal of Immunological Methods
Volume	33
Issue number	3
DOIs	https://doi.org/10.1016/0022-1759(80)90210-0
Publication status	Published - Apr 10 1980

Fingerprint

Leucine

Macrophages

Guanidine

Proteins

Cultured Cells

ASJC Scopus subject areas

Biotechnology
Immunology

Cite this

Varesio, L., & Eva, A. (1980). A microsystem to evaluate the synthesis of [3H]leucine labeled proteins by macrophages. Journal of Immunological Methods, 33(3), 231-238. https://doi.org/10.1016/0022-1759(80)90210-0

A microsystem to evaluate the synthesis of [3H]leucine labeled proteins by macrophages. / Varesio, Luigi ; Eva, Alessandra.

In: Journal of Immunological Methods, Vol. 33, No. 3, 10.04.1980, p. 231-238.

Research output: Contribution to journal › Article

Varesio, L & Eva, A 1980, 'A microsystem to evaluate the synthesis of [3H]leucine labeled proteins by macrophages', Journal of Immunological Methods, vol. 33, no. 3, pp. 231-238. https://doi.org/10.1016/0022-1759(80)90210-0

Varesio L , Eva A. A microsystem to evaluate the synthesis of [3H]leucine labeled proteins by macrophages. Journal of Immunological Methods. 1980 Apr 10;33(3):231-238. https://doi.org/10.1016/0022-1759(80)90210-0

Varesio, Luigi ; Eva, Alessandra. / A microsystem to evaluate the synthesis of [3H]leucine labeled proteins by macrophages. In: Journal of Immunological Methods. 1980 ; Vol. 33, No. 3. pp. 231-238.

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10.1016/0022-1759(80)90210-0