A microsystem to evaluate the synthesis of [3H]leucine labeled proteins by macrophages

Research output: Contribution to journalArticle

Abstract

A method is described for evaluating protein synthesis by adherent MF{cyrillic} by measuring the incorporation of [3H]leucine into TCA precipitable material. By using guanidine-HCl it was possible to remove completely the radiolabeled proteins from the adherent cells that were cultured in microwells, and retain TCA precipitable material. This procedure enabled us to harvest the TCA precipitable proteins with semiautomatic cell harvester. The guanidine-HCl treatment did not affect the recovery of the radioactive proteins and did not alter the sensitivity of the assay. This method is very simple and rapid and, since it is suitable for processing microcultures, permits detailed studies on the biology of small numbers of MF{cyrillic}.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalJournal of Immunological Methods
Volume33
Issue number3
DOIs
Publication statusPublished - Apr 10 1980

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Leucine
Macrophages
Guanidine
Proteins
Cultured Cells

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

A microsystem to evaluate the synthesis of [3H]leucine labeled proteins by macrophages. / Varesio, Luigi; Eva, Alessandra.

In: Journal of Immunological Methods, Vol. 33, No. 3, 10.04.1980, p. 231-238.

Research output: Contribution to journalArticle

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