TY - JOUR
T1 - A microsystem to evaluate the synthesis of [3H]leucine labeled proteins by macrophages
AU - Varesio, Luigi
AU - Eva, Alessandra
PY - 1980/4/10
Y1 - 1980/4/10
N2 - A method is described for evaluating protein synthesis by adherent MF{cyrillic} by measuring the incorporation of [3H]leucine into TCA precipitable material. By using guanidine-HCl it was possible to remove completely the radiolabeled proteins from the adherent cells that were cultured in microwells, and retain TCA precipitable material. This procedure enabled us to harvest the TCA precipitable proteins with semiautomatic cell harvester. The guanidine-HCl treatment did not affect the recovery of the radioactive proteins and did not alter the sensitivity of the assay. This method is very simple and rapid and, since it is suitable for processing microcultures, permits detailed studies on the biology of small numbers of MF{cyrillic}.
AB - A method is described for evaluating protein synthesis by adherent MF{cyrillic} by measuring the incorporation of [3H]leucine into TCA precipitable material. By using guanidine-HCl it was possible to remove completely the radiolabeled proteins from the adherent cells that were cultured in microwells, and retain TCA precipitable material. This procedure enabled us to harvest the TCA precipitable proteins with semiautomatic cell harvester. The guanidine-HCl treatment did not affect the recovery of the radioactive proteins and did not alter the sensitivity of the assay. This method is very simple and rapid and, since it is suitable for processing microcultures, permits detailed studies on the biology of small numbers of MF{cyrillic}.
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U2 - 10.1016/0022-1759(80)90210-0
DO - 10.1016/0022-1759(80)90210-0
M3 - Article
C2 - 6989918
AN - SCOPUS:0018849136
VL - 33
SP - 231
EP - 238
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 3
ER -