A miRNA signature in human cord blood stem and progenitor cells as potential biomarker of specific acute myeloid leukemia subtypes

M. Cattaneo, E. Pelosi, G. Castelli, A. M. Cerio, A. D'angiò, L. Porretti, P. Rebulla, L. Pavesi, G. Russo, A. Giordano, J. Turri, L. Cicconi, F. Lo-Coco, U. Testa, Ida Biunno

Research output: Contribution to journalArticle

Abstract

MicroRNAs (miRNAs) are important regulators of several cellular processes. During hematopoiesis, specific expression signatures have been reported in different blood cell lineages and stages of hematopoietic stem cell (HSC) differentiation. Here we explored the expression of miRNAs in umbilical cord blood stem (HSC) and progenitor cells (HPC) and compared it to unilineage granulocyte and granulo-monocyte differentiation as well as to primary blasts from patients with acute myeloid leukemia (AML). CD34+CD38- ad CD34+CD38+ cells were profiled using a global array consisting of about 2000 miRNAs. An approach combining bioinformatic prediction of miRNA targets with mRNA expression profiling was used to search for putative biologically enriched functions and networks. At least 15 miRNAs to be differentially expressed between HSC and HPC cell population, a cluster of 7 miRNAs are located in the q32 region of human chromosome 14 (miR-377-3p, -136-5p, 376a-3p, 495-3p, 654-3p, 376c-3p and 381-3p) whose expression decreased during the early stages of normal myelopoiesis but were markedly increased in a small set of AML. Interestingly, miR-4739 and -4516, two novel microRNA whose function and targets are presently unknown, showed specific and peculiar expression profile during the hematopoietic stem cells differentiation into unilineages and resulted strongly upregulated in almost all AML subsets. miR-181, -126-5p, -29b-3p and -22-3p resulted dis-regulated in specific leukemias phenotypes. This study provides the first evidence of a miRNA signature in human cord blood stem and progenitor cells with a potential role in hematopoietic stemness properties and possibly in leukemogenesis of specific AML subtypes. J. Cell. Physiol. 230: 1770-1780, 2015.

Original languageEnglish
Pages (from-to)1770-1780
Number of pages11
JournalJournal of Cellular Physiology
Volume230
Issue number8
DOIs
Publication statusPublished - Aug 1 2015

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Biomarkers
MicroRNAs
Fetal Blood
Acute Myeloid Leukemia
Blood Cells
Blood
Stem Cells
Hematopoietic Stem Cells
Stem cells
Cell Differentiation
Cells
Myelopoiesis
Chromosomes, Human, Pair 14
Hematopoiesis
Human Chromosomes
Cell Lineage
Bioinformatics
Chromosomes
Computational Biology
Granulocytes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Medicine(all)

Cite this

A miRNA signature in human cord blood stem and progenitor cells as potential biomarker of specific acute myeloid leukemia subtypes. / Cattaneo, M.; Pelosi, E.; Castelli, G.; Cerio, A. M.; D'angiò, A.; Porretti, L.; Rebulla, P.; Pavesi, L.; Russo, G.; Giordano, A.; Turri, J.; Cicconi, L.; Lo-Coco, F.; Testa, U.; Biunno, Ida.

In: Journal of Cellular Physiology, Vol. 230, No. 8, 01.08.2015, p. 1770-1780.

Research output: Contribution to journalArticle

Cattaneo, M, Pelosi, E, Castelli, G, Cerio, AM, D'angiò, A, Porretti, L, Rebulla, P, Pavesi, L, Russo, G, Giordano, A, Turri, J, Cicconi, L, Lo-Coco, F, Testa, U & Biunno, I 2015, 'A miRNA signature in human cord blood stem and progenitor cells as potential biomarker of specific acute myeloid leukemia subtypes', Journal of Cellular Physiology, vol. 230, no. 8, pp. 1770-1780. https://doi.org/10.1002/jcp.24876
Cattaneo, M. ; Pelosi, E. ; Castelli, G. ; Cerio, A. M. ; D'angiò, A. ; Porretti, L. ; Rebulla, P. ; Pavesi, L. ; Russo, G. ; Giordano, A. ; Turri, J. ; Cicconi, L. ; Lo-Coco, F. ; Testa, U. ; Biunno, Ida. / A miRNA signature in human cord blood stem and progenitor cells as potential biomarker of specific acute myeloid leukemia subtypes. In: Journal of Cellular Physiology. 2015 ; Vol. 230, No. 8. pp. 1770-1780.
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AU - Pelosi, E.

AU - Castelli, G.

AU - Cerio, A. M.

AU - D'angiò, A.

AU - Porretti, L.

AU - Rebulla, P.

AU - Pavesi, L.

AU - Russo, G.

AU - Giordano, A.

AU - Turri, J.

AU - Cicconi, L.

AU - Lo-Coco, F.

AU - Testa, U.

AU - Biunno, Ida

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N2 - MicroRNAs (miRNAs) are important regulators of several cellular processes. During hematopoiesis, specific expression signatures have been reported in different blood cell lineages and stages of hematopoietic stem cell (HSC) differentiation. Here we explored the expression of miRNAs in umbilical cord blood stem (HSC) and progenitor cells (HPC) and compared it to unilineage granulocyte and granulo-monocyte differentiation as well as to primary blasts from patients with acute myeloid leukemia (AML). CD34+CD38- ad CD34+CD38+ cells were profiled using a global array consisting of about 2000 miRNAs. An approach combining bioinformatic prediction of miRNA targets with mRNA expression profiling was used to search for putative biologically enriched functions and networks. At least 15 miRNAs to be differentially expressed between HSC and HPC cell population, a cluster of 7 miRNAs are located in the q32 region of human chromosome 14 (miR-377-3p, -136-5p, 376a-3p, 495-3p, 654-3p, 376c-3p and 381-3p) whose expression decreased during the early stages of normal myelopoiesis but were markedly increased in a small set of AML. Interestingly, miR-4739 and -4516, two novel microRNA whose function and targets are presently unknown, showed specific and peculiar expression profile during the hematopoietic stem cells differentiation into unilineages and resulted strongly upregulated in almost all AML subsets. miR-181, -126-5p, -29b-3p and -22-3p resulted dis-regulated in specific leukemias phenotypes. This study provides the first evidence of a miRNA signature in human cord blood stem and progenitor cells with a potential role in hematopoietic stemness properties and possibly in leukemogenesis of specific AML subtypes. J. Cell. Physiol. 230: 1770-1780, 2015.

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