A miRNome analysis of drug-free manic psychotic bipolar patients versus healthy controls

Silvia Tabano, Alice Caldiroli, Andrea Terrasi, Patrizia Colapietro, Silvia Grassi, Greta Silvia Carnevali, Laura Fontana, Marta Serati, Valentina Vaira, A. Carlo Altamura, Monica Miozzo, Massimiliano Buoli

Research output: Contribution to journalArticle

Abstract

The lifetime presence of psychotic symptoms is associated with more clinical severity, poorer outcome and biological changes in patients affected by bipolar disorder (BD). Epigenetic mechanisms have been evoked to explain the onset of psychotic symptoms in BD as well as the associated biological changes. The main objective of the present study was to evaluate the expression profiles of circulating microRNAs (miRNAs) in drug-free manic psychotic bipolar patients versus healthy controls (HC), to identify possible non-invasive molecular markers of the disorder. 15 drug-free manic psychotic bipolar patients and 9 HC were enrolled and 800 miRNAs expression profile was measured by Nanostring nCounter technology on plasma samples and validated through qPCR. Overall, twelve miRNAs showed a significantly altered expression between the two groups (p < 0.05). Functional annotation of predicted miRNAs targets by MultiMIR R tool showed repression in bipolar patients of genes with a role in neurodevelopment and neurogenesis, and upregulation of genes involved in metabolism regulation. We identified a signature of circulating miRNA characteristic of manic psychotic bipolar patients, suggesting a possible role in neurodevelopment and metabolic processes regulation.

Original languageEnglish
JournalEuropean Archives of Psychiatry and Clinical Neuroscience
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

MicroRNAs
Pharmaceutical Preparations
Bipolar Disorder
Neurogenesis
Epigenomics
Genes
Up-Regulation
Technology

Keywords

  • Biomarkers
  • Bipolar disorder (BD)
  • Epigenetics
  • Metabolism
  • miRNA
  • Psychotic symptoms

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Biological Psychiatry
  • Pharmacology (medical)

Cite this

@article{6927a9a03702407db2700bd709ec89a2,
title = "A miRNome analysis of drug-free manic psychotic bipolar patients versus healthy controls",
abstract = "The lifetime presence of psychotic symptoms is associated with more clinical severity, poorer outcome and biological changes in patients affected by bipolar disorder (BD). Epigenetic mechanisms have been evoked to explain the onset of psychotic symptoms in BD as well as the associated biological changes. The main objective of the present study was to evaluate the expression profiles of circulating microRNAs (miRNAs) in drug-free manic psychotic bipolar patients versus healthy controls (HC), to identify possible non-invasive molecular markers of the disorder. 15 drug-free manic psychotic bipolar patients and 9 HC were enrolled and 800 miRNAs expression profile was measured by Nanostring nCounter technology on plasma samples and validated through qPCR. Overall, twelve miRNAs showed a significantly altered expression between the two groups (p < 0.05). Functional annotation of predicted miRNAs targets by MultiMIR R tool showed repression in bipolar patients of genes with a role in neurodevelopment and neurogenesis, and upregulation of genes involved in metabolism regulation. We identified a signature of circulating miRNA characteristic of manic psychotic bipolar patients, suggesting a possible role in neurodevelopment and metabolic processes regulation.",
keywords = "Biomarkers, Bipolar disorder (BD), Epigenetics, Metabolism, miRNA, Psychotic symptoms",
author = "Silvia Tabano and Alice Caldiroli and Andrea Terrasi and Patrizia Colapietro and Silvia Grassi and Carnevali, {Greta Silvia} and Laura Fontana and Marta Serati and Valentina Vaira and Altamura, {A. Carlo} and Monica Miozzo and Massimiliano Buoli",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/s00406-019-01057-2",
language = "English",
journal = "European Archives of Psychiatry and Clinical Neuroscience",
issn = "0940-1334",
publisher = "Springer Verlag",

}

TY - JOUR

T1 - A miRNome analysis of drug-free manic psychotic bipolar patients versus healthy controls

AU - Tabano, Silvia

AU - Caldiroli, Alice

AU - Terrasi, Andrea

AU - Colapietro, Patrizia

AU - Grassi, Silvia

AU - Carnevali, Greta Silvia

AU - Fontana, Laura

AU - Serati, Marta

AU - Vaira, Valentina

AU - Altamura, A. Carlo

AU - Miozzo, Monica

AU - Buoli, Massimiliano

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The lifetime presence of psychotic symptoms is associated with more clinical severity, poorer outcome and biological changes in patients affected by bipolar disorder (BD). Epigenetic mechanisms have been evoked to explain the onset of psychotic symptoms in BD as well as the associated biological changes. The main objective of the present study was to evaluate the expression profiles of circulating microRNAs (miRNAs) in drug-free manic psychotic bipolar patients versus healthy controls (HC), to identify possible non-invasive molecular markers of the disorder. 15 drug-free manic psychotic bipolar patients and 9 HC were enrolled and 800 miRNAs expression profile was measured by Nanostring nCounter technology on plasma samples and validated through qPCR. Overall, twelve miRNAs showed a significantly altered expression between the two groups (p < 0.05). Functional annotation of predicted miRNAs targets by MultiMIR R tool showed repression in bipolar patients of genes with a role in neurodevelopment and neurogenesis, and upregulation of genes involved in metabolism regulation. We identified a signature of circulating miRNA characteristic of manic psychotic bipolar patients, suggesting a possible role in neurodevelopment and metabolic processes regulation.

AB - The lifetime presence of psychotic symptoms is associated with more clinical severity, poorer outcome and biological changes in patients affected by bipolar disorder (BD). Epigenetic mechanisms have been evoked to explain the onset of psychotic symptoms in BD as well as the associated biological changes. The main objective of the present study was to evaluate the expression profiles of circulating microRNAs (miRNAs) in drug-free manic psychotic bipolar patients versus healthy controls (HC), to identify possible non-invasive molecular markers of the disorder. 15 drug-free manic psychotic bipolar patients and 9 HC were enrolled and 800 miRNAs expression profile was measured by Nanostring nCounter technology on plasma samples and validated through qPCR. Overall, twelve miRNAs showed a significantly altered expression between the two groups (p < 0.05). Functional annotation of predicted miRNAs targets by MultiMIR R tool showed repression in bipolar patients of genes with a role in neurodevelopment and neurogenesis, and upregulation of genes involved in metabolism regulation. We identified a signature of circulating miRNA characteristic of manic psychotic bipolar patients, suggesting a possible role in neurodevelopment and metabolic processes regulation.

KW - Biomarkers

KW - Bipolar disorder (BD)

KW - Epigenetics

KW - Metabolism

KW - miRNA

KW - Psychotic symptoms

UR - http://www.scopus.com/inward/record.url?scp=85070821445&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070821445&partnerID=8YFLogxK

U2 - 10.1007/s00406-019-01057-2

DO - 10.1007/s00406-019-01057-2

M3 - Article

AN - SCOPUS:85070821445

JO - European Archives of Psychiatry and Clinical Neuroscience

JF - European Archives of Psychiatry and Clinical Neuroscience

SN - 0940-1334

ER -