A modified vimentin histological score helps recognize pulmonary sarcomatoid carcinoma in small biopsy samples

Giuseppe Pelosi, Flavia Melotti, Alberto Cavazza, Giulio Rossi, Patrick Maisonneuve, Paolo Graziano, Mattia Barbareschi, Yukio Nakatani, Mauro Papotti

Research output: Contribution to journalArticlepeer-review

Abstract

Background: As pulmonary sarcomatoid carcinomas (PSCs) are life-threatening tumors, an improvement in their recognition in small-sized tumor samples is clinically warranted. Materials and Methods: Preoperative biopsy samples and paired surgical specimens from 20 pleomorphic carcinomas, two pulmonary blastemas and one carcinosarcoma (training set) were studied for vimentin immunohistochemistry. A modified vimentin histologic score (M-VHS) was devised by multiplying three independently assessed parameters, i.e. the percentage of positive cells (from 0 to 5+, by quintiles), the intensity of immunostaining (low=1 vs. strong=2) and the distribution pattern within the cytoplasm (partial=1 vs. diffuse=2), so ranging from 0 to 20. Forty-eight consecutive and independent cases of non-small cell lung carcinoma (NSCLC), including two additional cases of PSC, were used as control groups (validation set). Results: No differences in M-VHS were found between biopsies and surgical specimens of PSC, thus confirming the occurrence of stable epithelial mesenchymal transition (EMT) and hence the specific diagnosis of PSC. All types of PSC shared the same M-VHS. The M-VHS of 46 conventional NSCLC was by far lower (p

Original languageEnglish
Pages (from-to)1463-1473
Number of pages11
JournalAnticancer Research
Volume32
Issue number4
Publication statusPublished - Apr 2012

Keywords

  • Biopsy
  • Cytokeratins
  • Immunohistochemistry
  • Lung cancer
  • Morphology
  • p40
  • p63
  • Pulmonary sarcomatoid carcinoma
  • TTF1
  • Vimentin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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