A monoclonal antibody specific for prophase phosphorylation of histone deacetylase 1: A readout for early mitotic cells

Chiara V. Segréy, Silvia Senesey, Sara Lopontey, Stefano Santaguida, Paolo Soffientini, Gabriela Grigorean, Mario Cinquanta, Giuseppe Ossolengo, Christian Seiser, Susanna Chiocca

Research output: Contribution to journalArticle

Abstract

Histone deacetylases (HDACs) are modification enzymes that regulate a plethora of biological processes. HDAC1, a crucial epigenetic modifier, is deregulated in cancer and subjected to a variety of post-translational modifications. Here, we describe the generation of a new monoclonal antibody that specifically recognizes a novel highly dynamic prophase phosphorylation of serine 406-HDAC1, providing a powerful tool for detecting early mitotic cells.

Original languageEnglish
Pages (from-to)37-42
Number of pages6
JournalmAbs
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

Keywords

  • Antibody
  • Cell cycle
  • Checkpoint
  • HDAC1
  • HDAC2
  • HDACs
  • Mitosis
  • Phosphorylation
  • Post-translational modifications
  • Prophase

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'A monoclonal antibody specific for prophase phosphorylation of histone deacetylase 1: A readout for early mitotic cells'. Together they form a unique fingerprint.

  • Cite this

    Segréy, C. V., Senesey, S., Lopontey, S., Santaguida, S., Soffientini, P., Grigorean, G., Cinquanta, M., Ossolengo, G., Seiser, C., & Chiocca, S. (2016). A monoclonal antibody specific for prophase phosphorylation of histone deacetylase 1: A readout for early mitotic cells. mAbs, 8(1), 37-42. https://doi.org/10.1080/19420862.2015.1098795