A monoclonal antibody to antigens expressed on MLR-activated T cells inhibits granulocyte macrophage colony formation

A. Bacigalupo, M. Podesta, F. Frassoni, A. Marmont, M. Risso, G. Corte

Research output: Contribution to journalArticlepeer-review

Abstract

A monoclonal antibody specifically reactive with MLR-activated T cells (MLR2) was added to light density normal marrow cells, depleted of adherent cells and T lymphocytes, and plated in soft agar for granulocyte macrophage colony formation. Colonies from MLR2-treated marrow cells were reduced to less than 10% of expected growth. The inhibition was not complement dependent, did not require the continuous presence of MLR2 in culture, and could not be detected also when human placenta-conditioned medium was used in the place of leukocyte feeder layers as a source of colony-stimulating factor (CSF). Co-culture experiments with MLR2 treated and untreated marrow cells further excluded the possibility of an indirect effect of MLR2 on CFU-c via auxiliary cells. The results of this study suggest that myeloid progenitor cells express a lymphoid antigen that is absent on resting or activated B cells and on resting T cells, but is expressed on activated T cells.

Original languageEnglish
Pages (from-to)2415-2417
Number of pages3
JournalJournal of Immunology
Volume127
Issue number6
Publication statusPublished - 1981

ASJC Scopus subject areas

  • Immunology

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