A monoclonal antibody to antigens expressed on MLR-activated T cells inhibits granulocyte macrophage colony formation

A. Bacigalupo, M. Podesta, F. Frassoni, A. Marmont, M. Risso, G. Corte

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A monoclonal antibody specifically reactive with MLR-activated T cells (MLR2) was added to light density normal marrow cells, depleted of adherent cells and T lymphocytes, and plated in soft agar for granulocyte macrophage colony formation. Colonies from MLR2-treated marrow cells were reduced to less than 10% of expected growth. The inhibition was not complement dependent, did not require the continuous presence of MLR2 in culture, and could not be detected also when human placenta-conditioned medium was used in the place of leukocyte feeder layers as a source of colony-stimulating factor (CSF). Co-culture experiments with MLR2 treated and untreated marrow cells further excluded the possibility of an indirect effect of MLR2 on CFU-c via auxiliary cells. The results of this study suggest that myeloid progenitor cells express a lymphoid antigen that is absent on resting or activated B cells and on resting T cells, but is expressed on activated T cells.

Original languageEnglish
Pages (from-to)2415-2417
Number of pages3
JournalJournal of Immunology
Volume127
Issue number6
Publication statusPublished - 1981

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Granulocytes
Macrophages
Monoclonal Antibodies
T-Lymphocytes
Antigens
Bone Marrow
Myeloid Progenitor Cells
Feeder Cells
Colony-Stimulating Factors
Conditioned Culture Medium
Coculture Techniques
Placenta
Agar
Leukocytes
B-Lymphocytes
Light
Growth

ASJC Scopus subject areas

  • Immunology

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A monoclonal antibody to antigens expressed on MLR-activated T cells inhibits granulocyte macrophage colony formation. / Bacigalupo, A.; Podesta, M.; Frassoni, F.; Marmont, A.; Risso, M.; Corte, G.

In: Journal of Immunology, Vol. 127, No. 6, 1981, p. 2415-2417.

Research output: Contribution to journalArticle

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AU - Podesta, M.

AU - Frassoni, F.

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AU - Risso, M.

AU - Corte, G.

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