A monoclonal antibody to platelet glycoproteins IIb and IIIa complexes: Its use in purifying human megakaryocytes from sternal bone marrow aspirates for immunofluorescence studies of Ia-like antigens

G. Damiani, E. Zocchi, M. Fabbi, A. Bargellesi, F. Patrone

Research output: Contribution to journalArticlepeer-review

Abstract

A monoclonal antibody (PBM 6.4) to platelet and metakaryocyte glycoproteins IIb and IIIa has been obtained and used to purify human megakaryocytes from sternal bone marrow aspirates by a simple method, consisting of a Percoll gradient centrifugation followed by affinity adherence on PBM 6.4-coated plastic surfaces, 'panning'. Megakaryocytes, 80-90% pure and morphologically well preserved, were attached to poly-L-ornithine-coated multi-well microscope slides and immunofluorescence was done using monoclonal antibodies to human Ia-like antigens (DR, DC1). A higher proportion of DR-positive megakaryocytes was found, in comparison to the values reported by others, while DC1 antigen was detected on about 20% of megakarocytes. The method described has the unique feature of enriching cells of the human megakaryocytic lineage from simple diagnostic sternal aspirates in an amount adequate for immunofluorescent and morphological analysis.

Original languageEnglish
Pages (from-to)169-177
Number of pages9
JournalExperimental Hematology
Volume11
Issue number3
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

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