A monoclonal antibody to platelet glycoproteins IIb and IIIa complexes: Its use in purifying human megakaryocytes from sternal bone marrow aspirates for immunofluorescence studies of Ia-like antigens

G. Damiani, E. Zocchi, M. Fabbi, A. Bargellesi, F. Patrone

Research output: Contribution to journalArticle

Abstract

A monoclonal antibody (PBM 6.4) to platelet and metakaryocyte glycoproteins IIb and IIIa has been obtained and used to purify human megakaryocytes from sternal bone marrow aspirates by a simple method, consisting of a Percoll gradient centrifugation followed by affinity adherence on PBM 6.4-coated plastic surfaces, 'panning'. Megakaryocytes, 80-90% pure and morphologically well preserved, were attached to poly-L-ornithine-coated multi-well microscope slides and immunofluorescence was done using monoclonal antibodies to human Ia-like antigens (DR, DC1). A higher proportion of DR-positive megakaryocytes was found, in comparison to the values reported by others, while DC1 antigen was detected on about 20% of megakarocytes. The method described has the unique feature of enriching cells of the human megakaryocytic lineage from simple diagnostic sternal aspirates in an amount adequate for immunofluorescent and morphological analysis.

Original languageEnglish
Pages (from-to)169-177
Number of pages9
JournalExperimental Hematology
Volume11
Issue number3
Publication statusPublished - 1983

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Integrin beta3
Platelet Glycoprotein GPIIb-IIIa Complex
Megakaryocytes
Histocompatibility Antigens Class II
Fluorescent Antibody Technique
Bone Marrow
Monoclonal Antibodies
HLA-D Antigens
Centrifugation
Plastics
Antigens
phytobacteriomycin

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

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title = "A monoclonal antibody to platelet glycoproteins IIb and IIIa complexes: Its use in purifying human megakaryocytes from sternal bone marrow aspirates for immunofluorescence studies of Ia-like antigens",
abstract = "A monoclonal antibody (PBM 6.4) to platelet and metakaryocyte glycoproteins IIb and IIIa has been obtained and used to purify human megakaryocytes from sternal bone marrow aspirates by a simple method, consisting of a Percoll gradient centrifugation followed by affinity adherence on PBM 6.4-coated plastic surfaces, 'panning'. Megakaryocytes, 80-90{\%} pure and morphologically well preserved, were attached to poly-L-ornithine-coated multi-well microscope slides and immunofluorescence was done using monoclonal antibodies to human Ia-like antigens (DR, DC1). A higher proportion of DR-positive megakaryocytes was found, in comparison to the values reported by others, while DC1 antigen was detected on about 20{\%} of megakarocytes. The method described has the unique feature of enriching cells of the human megakaryocytic lineage from simple diagnostic sternal aspirates in an amount adequate for immunofluorescent and morphological analysis.",
author = "G. Damiani and E. Zocchi and M. Fabbi and A. Bargellesi and F. Patrone",
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T1 - A monoclonal antibody to platelet glycoproteins IIb and IIIa complexes

T2 - Its use in purifying human megakaryocytes from sternal bone marrow aspirates for immunofluorescence studies of Ia-like antigens

AU - Damiani, G.

AU - Zocchi, E.

AU - Fabbi, M.

AU - Bargellesi, A.

AU - Patrone, F.

PY - 1983

Y1 - 1983

N2 - A monoclonal antibody (PBM 6.4) to platelet and metakaryocyte glycoproteins IIb and IIIa has been obtained and used to purify human megakaryocytes from sternal bone marrow aspirates by a simple method, consisting of a Percoll gradient centrifugation followed by affinity adherence on PBM 6.4-coated plastic surfaces, 'panning'. Megakaryocytes, 80-90% pure and morphologically well preserved, were attached to poly-L-ornithine-coated multi-well microscope slides and immunofluorescence was done using monoclonal antibodies to human Ia-like antigens (DR, DC1). A higher proportion of DR-positive megakaryocytes was found, in comparison to the values reported by others, while DC1 antigen was detected on about 20% of megakarocytes. The method described has the unique feature of enriching cells of the human megakaryocytic lineage from simple diagnostic sternal aspirates in an amount adequate for immunofluorescent and morphological analysis.

AB - A monoclonal antibody (PBM 6.4) to platelet and metakaryocyte glycoproteins IIb and IIIa has been obtained and used to purify human megakaryocytes from sternal bone marrow aspirates by a simple method, consisting of a Percoll gradient centrifugation followed by affinity adherence on PBM 6.4-coated plastic surfaces, 'panning'. Megakaryocytes, 80-90% pure and morphologically well preserved, were attached to poly-L-ornithine-coated multi-well microscope slides and immunofluorescence was done using monoclonal antibodies to human Ia-like antigens (DR, DC1). A higher proportion of DR-positive megakaryocytes was found, in comparison to the values reported by others, while DC1 antigen was detected on about 20% of megakarocytes. The method described has the unique feature of enriching cells of the human megakaryocytic lineage from simple diagnostic sternal aspirates in an amount adequate for immunofluorescent and morphological analysis.

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