A monte carlo study of the radiation quality dependence of DNA fragmentation spectra

D. Alloni, A. Campa, M. Belli, G. Esposito, A. Facoetti, W. Friedland, M. Liotta, L. Mariotti, H. G. Paretzke, A. Ottolenghi

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We simulated the irradiation of human fibroblasts with γ rays, protons and helium, carbon and iron ions at a fixed dose of 5 Gy. The simulations were performed with the biophysical Monte Carlo code PARTRAC. From the output of the code, containing in particular the genomic positions of the radiation-induced DNA double-strand breaks (DSBs), we obtained the DNA fragmentation spectra. Very small fragments, in particular those related to "complex lesions" (few tens of base pairs), are probably very important for the late cellular consequences, but their detection is not possible with the common experimental techniques. We paid special attention to the differences among the various ions in the production of these very small fragments; in particular, we compared the fragmentation spectra for ions of the same specific energy and for ions of the same LET (linear energy transfer). As found previously for iron ions, we found that the RBE (relative biological effectiveness) for DSB production was considerably higher than 1 for all high-LET radiations considered. This is at variance with the results obtainable from experimental data, and it is due to the ability to count the contribution of small fragments. It should be noted that for a given LET this RBE decreases with increasing ion charge, due mainly to the increasing mean energy of secondary electrons. A precise quantification of the DNA initial damage can be of great importance for both radiation protection, particularly in open-space long-term manned missions, and hadrontherapy.

Original languageEnglish
Pages (from-to)263-271
Number of pages9
JournalRadiation Research
Volume173
Issue number3
DOIs
Publication statusPublished - Mar 2010

Fingerprint

DNA Fragmentation
fragmentation
deoxyribonucleic acid
Ions
Radiation
Linear Energy Transfer
linear energy transfer (LET)
radiation
Relative Biological Effectiveness
ions
fragments
strands
Iron
iron
Helium
Radiation Protection
ion charge
Double-Stranded DNA Breaks
helium ions
radiation protection

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Alloni, D., Campa, A., Belli, M., Esposito, G., Facoetti, A., Friedland, W., ... Ottolenghi, A. (2010). A monte carlo study of the radiation quality dependence of DNA fragmentation spectra. Radiation Research, 173(3), 263-271. https://doi.org/10.1667/RR1957.1

A monte carlo study of the radiation quality dependence of DNA fragmentation spectra. / Alloni, D.; Campa, A.; Belli, M.; Esposito, G.; Facoetti, A.; Friedland, W.; Liotta, M.; Mariotti, L.; Paretzke, H. G.; Ottolenghi, A.

In: Radiation Research, Vol. 173, No. 3, 03.2010, p. 263-271.

Research output: Contribution to journalArticle

Alloni, D, Campa, A, Belli, M, Esposito, G, Facoetti, A, Friedland, W, Liotta, M, Mariotti, L, Paretzke, HG & Ottolenghi, A 2010, 'A monte carlo study of the radiation quality dependence of DNA fragmentation spectra', Radiation Research, vol. 173, no. 3, pp. 263-271. https://doi.org/10.1667/RR1957.1
Alloni D, Campa A, Belli M, Esposito G, Facoetti A, Friedland W et al. A monte carlo study of the radiation quality dependence of DNA fragmentation spectra. Radiation Research. 2010 Mar;173(3):263-271. https://doi.org/10.1667/RR1957.1
Alloni, D. ; Campa, A. ; Belli, M. ; Esposito, G. ; Facoetti, A. ; Friedland, W. ; Liotta, M. ; Mariotti, L. ; Paretzke, H. G. ; Ottolenghi, A. / A monte carlo study of the radiation quality dependence of DNA fragmentation spectra. In: Radiation Research. 2010 ; Vol. 173, No. 3. pp. 263-271.
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