A morphological analysis endocrine tumour genesis in pancreas and anterior pituitary of AVP/SV40 transgenic mice

G. Rindi, A. E. Bishop, D. Murphy, E. Solcia, B. Hogan, J. M. Polak

Research output: Contribution to journalArticle

Abstract

Insertion into the mouse genome of the hybrid oncogene made up of bovine vasopressin gene derived 5′ upstream sequences and the coding sequences of SV40 large T-antigen promoted tumours in anterior pituitary and endocrine pancreas of mice bearing this transgene. In order to investigate the morphology of the steps in the neoplastic process, we used light and electron microscopy to study these organs in 42 animals belonging to the 3rd, 4th and 5th generations, subdivided into 4 age groups from 20 days to 100 days of life. Antibodies to large T-antigen were used to identify sites of expression of the hybrid oncogene, thus monitoring the steps in neoplastic transformation. Large T-antigen immunoreactivity was identified in dysplastic lesions of younger animals and in both dysplastic lesions and tumours of older mice. Insulin (100% of cases) and pancreatic polypeptide (25% of cases) immunoreactivities were revealed in pancreatic lesions but no hormonal immunoreactivity was detected in the pituitary lesions. The ultrastructural study confirmed that the majority cell population of the pancreatic neoplasms was B-type and that the anterior pituitary tumours were poorly granulated. The subcellular localization of large T-antigen immunoreactivity was investigated by the immunogold method and was confined to the heterochromatin of tumour cell nuclei. These findings provide evidence for the dysplasia-neoplasia sequence in the genesis of endocrine tumours of pituitary and pancreas of transgenic mice. The vasopressin-SV40 large T-antigen transgenic mice may therefore be an useful model for the study of endocrine cell oncogenesis,

Original languageEnglish
Pages (from-to)255-266
Number of pages12
JournalVirchows Archiv A Pathological Anatomy and Histopathology
Volume412
Issue number3
DOIs
Publication statusPublished - May 1988

Fingerprint

Viral Tumor Antigens
Transgenic Mice
Pancreas
Polyomavirus Transforming Antigens
Pituitary Neoplasms
Neoplasms
Vasopressins
Oncogenes
Neoplastic Processes
Pancreatic Polypeptide
Endocrine Cells
Heterochromatin
Cell Nucleus
Pancreatic Neoplasms
Transgenes
Islets of Langerhans
Electron Microscopy
Carcinogenesis
Age Groups
Genome

Keywords

  • AVP/SV40 transgenic mice
  • Dysplasia
  • Heterochromatin
  • Immunocytochemistry
  • Insulin
  • Large T-antigen
  • Neoplasia
  • Pancreatic polypeptide

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

Cite this

A morphological analysis endocrine tumour genesis in pancreas and anterior pituitary of AVP/SV40 transgenic mice. / Rindi, G.; Bishop, A. E.; Murphy, D.; Solcia, E.; Hogan, B.; Polak, J. M.

In: Virchows Archiv A Pathological Anatomy and Histopathology, Vol. 412, No. 3, 05.1988, p. 255-266.

Research output: Contribution to journalArticle

@article{7bfc6656297a476792f7a1e780e73bf2,
title = "A morphological analysis endocrine tumour genesis in pancreas and anterior pituitary of AVP/SV40 transgenic mice",
abstract = "Insertion into the mouse genome of the hybrid oncogene made up of bovine vasopressin gene derived 5′ upstream sequences and the coding sequences of SV40 large T-antigen promoted tumours in anterior pituitary and endocrine pancreas of mice bearing this transgene. In order to investigate the morphology of the steps in the neoplastic process, we used light and electron microscopy to study these organs in 42 animals belonging to the 3rd, 4th and 5th generations, subdivided into 4 age groups from 20 days to 100 days of life. Antibodies to large T-antigen were used to identify sites of expression of the hybrid oncogene, thus monitoring the steps in neoplastic transformation. Large T-antigen immunoreactivity was identified in dysplastic lesions of younger animals and in both dysplastic lesions and tumours of older mice. Insulin (100{\%} of cases) and pancreatic polypeptide (25{\%} of cases) immunoreactivities were revealed in pancreatic lesions but no hormonal immunoreactivity was detected in the pituitary lesions. The ultrastructural study confirmed that the majority cell population of the pancreatic neoplasms was B-type and that the anterior pituitary tumours were poorly granulated. The subcellular localization of large T-antigen immunoreactivity was investigated by the immunogold method and was confined to the heterochromatin of tumour cell nuclei. These findings provide evidence for the dysplasia-neoplasia sequence in the genesis of endocrine tumours of pituitary and pancreas of transgenic mice. The vasopressin-SV40 large T-antigen transgenic mice may therefore be an useful model for the study of endocrine cell oncogenesis,",
keywords = "AVP/SV40 transgenic mice, Dysplasia, Heterochromatin, Immunocytochemistry, Insulin, Large T-antigen, Neoplasia, Pancreatic polypeptide",
author = "G. Rindi and Bishop, {A. E.} and D. Murphy and E. Solcia and B. Hogan and Polak, {J. M.}",
year = "1988",
month = "5",
doi = "10.1007/BF00737150",
language = "English",
volume = "412",
pages = "255--266",
journal = "Virchows Archiv - A Pathological Anatomy and Histopathology",
issn = "0945-6317",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - A morphological analysis endocrine tumour genesis in pancreas and anterior pituitary of AVP/SV40 transgenic mice

AU - Rindi, G.

AU - Bishop, A. E.

AU - Murphy, D.

AU - Solcia, E.

AU - Hogan, B.

AU - Polak, J. M.

PY - 1988/5

Y1 - 1988/5

N2 - Insertion into the mouse genome of the hybrid oncogene made up of bovine vasopressin gene derived 5′ upstream sequences and the coding sequences of SV40 large T-antigen promoted tumours in anterior pituitary and endocrine pancreas of mice bearing this transgene. In order to investigate the morphology of the steps in the neoplastic process, we used light and electron microscopy to study these organs in 42 animals belonging to the 3rd, 4th and 5th generations, subdivided into 4 age groups from 20 days to 100 days of life. Antibodies to large T-antigen were used to identify sites of expression of the hybrid oncogene, thus monitoring the steps in neoplastic transformation. Large T-antigen immunoreactivity was identified in dysplastic lesions of younger animals and in both dysplastic lesions and tumours of older mice. Insulin (100% of cases) and pancreatic polypeptide (25% of cases) immunoreactivities were revealed in pancreatic lesions but no hormonal immunoreactivity was detected in the pituitary lesions. The ultrastructural study confirmed that the majority cell population of the pancreatic neoplasms was B-type and that the anterior pituitary tumours were poorly granulated. The subcellular localization of large T-antigen immunoreactivity was investigated by the immunogold method and was confined to the heterochromatin of tumour cell nuclei. These findings provide evidence for the dysplasia-neoplasia sequence in the genesis of endocrine tumours of pituitary and pancreas of transgenic mice. The vasopressin-SV40 large T-antigen transgenic mice may therefore be an useful model for the study of endocrine cell oncogenesis,

AB - Insertion into the mouse genome of the hybrid oncogene made up of bovine vasopressin gene derived 5′ upstream sequences and the coding sequences of SV40 large T-antigen promoted tumours in anterior pituitary and endocrine pancreas of mice bearing this transgene. In order to investigate the morphology of the steps in the neoplastic process, we used light and electron microscopy to study these organs in 42 animals belonging to the 3rd, 4th and 5th generations, subdivided into 4 age groups from 20 days to 100 days of life. Antibodies to large T-antigen were used to identify sites of expression of the hybrid oncogene, thus monitoring the steps in neoplastic transformation. Large T-antigen immunoreactivity was identified in dysplastic lesions of younger animals and in both dysplastic lesions and tumours of older mice. Insulin (100% of cases) and pancreatic polypeptide (25% of cases) immunoreactivities were revealed in pancreatic lesions but no hormonal immunoreactivity was detected in the pituitary lesions. The ultrastructural study confirmed that the majority cell population of the pancreatic neoplasms was B-type and that the anterior pituitary tumours were poorly granulated. The subcellular localization of large T-antigen immunoreactivity was investigated by the immunogold method and was confined to the heterochromatin of tumour cell nuclei. These findings provide evidence for the dysplasia-neoplasia sequence in the genesis of endocrine tumours of pituitary and pancreas of transgenic mice. The vasopressin-SV40 large T-antigen transgenic mice may therefore be an useful model for the study of endocrine cell oncogenesis,

KW - AVP/SV40 transgenic mice

KW - Dysplasia

KW - Heterochromatin

KW - Immunocytochemistry

KW - Insulin

KW - Large T-antigen

KW - Neoplasia

KW - Pancreatic polypeptide

UR - http://www.scopus.com/inward/record.url?scp=0023870769&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023870769&partnerID=8YFLogxK

U2 - 10.1007/BF00737150

DO - 10.1007/BF00737150

M3 - Article

C2 - 2829418

AN - SCOPUS:0023870769

VL - 412

SP - 255

EP - 266

JO - Virchows Archiv - A Pathological Anatomy and Histopathology

JF - Virchows Archiv - A Pathological Anatomy and Histopathology

SN - 0945-6317

IS - 3

ER -