A mos oncogene-containing retrovirus, myeloproliferative sarcoma virus, transforms rat thyroid epithelial cells and irreversibly blocks their differentiation pattern

A. Fusco, G. Portella, P. P. Di Fiore, M. T. Berlingieri, R. Di Lauro, A. B. Schneider, G. Vecchio

Research output: Contribution to journalArticle

Abstract

Differentiated, cloned rat thyroid epithelial cells (424 cells) were infected with a wild-type and a temperature-sensitive strain of the myeloproliferative variant of the Moloney murine sarcoma virus. The thyroid cells were productively infected and transformed by both virus strains and displayed some of the typical properties of malignant cells, such as morphological changes, growth in soft agar, and in vivo tumorigenicity. The acquisition of the transformed phenotype by the virus-infected cells was accompanied by a loss of the typical differentiated features of the thyroid epithelium, such as thyroglobulin (TG) secretion, iodide uptake, and dependence for growth on six factors including thyrotropin, the physiological thyroid stimulator. TG mRNA could not be demonstrated in cells transformed by both viral strains, suggesting a block at the level of the TG gene transcription. While the transformed state of the cell clones infected with the temperature-sensitive strain could be reverted by shifting the cultures to the temperature nonpermissive for transformation (39°C), no reversion of the differential functions took place after such a shift, showing that the v-mos oncogene irreversibly shuts off the differentiation of thyroid epithelial cells in vitro. These results demonstrate, for the first time, an oncogenic potential of the v-mos oncogene family towards differentiated epithelial cells in vitro.

Original languageEnglish
Pages (from-to)284-292
Number of pages9
JournalJournal of Virology
Volume56
Issue number1
Publication statusPublished - 1985

Fingerprint

mos Genes
Retroviridae
Parvovirus
oncogenes
sarcoma
Sarcoma
epithelial cells
Thyroglobulin
thyroglobulin
viruses
rats
Thyroid Gland
Temperature
cells
Moloney murine sarcoma virus
Viruses
Iodides
Thyrotropin
Growth
temperature

ASJC Scopus subject areas

  • Immunology

Cite this

A mos oncogene-containing retrovirus, myeloproliferative sarcoma virus, transforms rat thyroid epithelial cells and irreversibly blocks their differentiation pattern. / Fusco, A.; Portella, G.; Di Fiore, P. P.; Berlingieri, M. T.; Di Lauro, R.; Schneider, A. B.; Vecchio, G.

In: Journal of Virology, Vol. 56, No. 1, 1985, p. 284-292.

Research output: Contribution to journalArticle

Fusco, A. ; Portella, G. ; Di Fiore, P. P. ; Berlingieri, M. T. ; Di Lauro, R. ; Schneider, A. B. ; Vecchio, G. / A mos oncogene-containing retrovirus, myeloproliferative sarcoma virus, transforms rat thyroid epithelial cells and irreversibly blocks their differentiation pattern. In: Journal of Virology. 1985 ; Vol. 56, No. 1. pp. 284-292.
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AU - Di Lauro, R.

AU - Schneider, A. B.

AU - Vecchio, G.

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