A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes

Fabio Maiullari, Marco Costantini, Marika Milan, Valentina Pace, Maila Chirivì, Silvia Maiullari, Alberto Rainer, Denisa Baci, Hany El-Sayed Marei, Dror Seliktar, Cesare Gargioli, Claudia Bearzi, Roberto Rizzi

Research output: Contribution to journalArticle

Abstract

The myocardium behaves like a sophisticated orchestra that expresses its true potential only if each member performs the correct task harmonically. Recapitulating its complexity within engineered 3D functional constructs with tailored biological and mechanical properties, is one of the current scientific priorities in the field of regenerative medicine and tissue engineering. In this study, driven by the necessity of fabricating advanced model of cardiac tissue, we present an innovative approach consisting of heterogeneous, multi-cellular constructs composed of Human Umbilical Vein Endothelial Cells (HUVECs) and induced pluripotent cell-derived cardiomyocytes (iPSC-CMs). Cells were encapsulated within hydrogel strands containing alginate and PEG-Fibrinogen (PF) and extruded through a custom microfluidic printing head (MPH) that allows to precisely tailor their 3D spatial deposition, guaranteeing a high printing fidelity and resolution. We obtained a 3D cardiac tissue compose of iPSC-derived CMs with a high orientation index imposed by the different defined geometries and blood vessel-like shapes generated by HUVECs which, as demonstrated by in vivo grafting, better support the integration of the engineered cardiac tissue with host's vasculature.

Original languageEnglish
Pages (from-to)13532
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Sep 10 2018

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Bioprinting
Human Umbilical Vein Endothelial Cells
Tissue Engineering
Cardiac Myocytes
Printing
Regenerative Medicine
Microfluidics
Hydrogel
Fibrinogen
Blood Vessels
Myocardium
Head

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A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes. / Maiullari, Fabio; Costantini, Marco; Milan, Marika; Pace, Valentina; Chirivì, Maila; Maiullari, Silvia; Rainer, Alberto; Baci, Denisa; Marei, Hany El-Sayed; Seliktar, Dror; Gargioli, Cesare; Bearzi, Claudia; Rizzi, Roberto.

In: Scientific Reports, Vol. 8, No. 1, 10.09.2018, p. 13532.

Research output: Contribution to journalArticle

Maiullari, F, Costantini, M, Milan, M, Pace, V, Chirivì, M, Maiullari, S, Rainer, A, Baci, D, Marei, HE-S, Seliktar, D, Gargioli, C, Bearzi, C & Rizzi, R 2018, 'A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes', Scientific Reports, vol. 8, no. 1, pp. 13532. https://doi.org/10.1038/s41598-018-31848-x
Maiullari, Fabio ; Costantini, Marco ; Milan, Marika ; Pace, Valentina ; Chirivì, Maila ; Maiullari, Silvia ; Rainer, Alberto ; Baci, Denisa ; Marei, Hany El-Sayed ; Seliktar, Dror ; Gargioli, Cesare ; Bearzi, Claudia ; Rizzi, Roberto. / A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes. In: Scientific Reports. 2018 ; Vol. 8, No. 1. pp. 13532.
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