A multimeric immunogen for the induction of immune memory to beta-amyloid

Francesca Mantile, Carla Basile, Valeria Cicatiello, Diana De Falco, Antonella Caivano, Piergiuseppe De Berardinis, Antonella Prisco

Research output: Contribution to journalArticle

Abstract

The development of active immunotherapy for Alzheimer's disease (AD) requires the identification of immunogens that can ensure a high titer antibody response toward beta-amyloid, whereas minimizing the risks of a cell-mediated adverse reaction. We describe here two novel anti-beta-amyloid vaccines that consist of virus like particles formed by a domain of the bacterial protein E2 that is able to self-assemble into a 60-mer peptide. Peptides 1-11 and 2-6 of beta-amyloid were displayed as N terminal fusions on the surface of the E2 particles. E2-based vaccines induced a fast-rising, robust and persistent antibody response to beta-amyloid in all vaccinated mice. The immune memory induced by a single administration of vaccine (1-11) E2 can be rapidly mobilized by a single booster injection, leading to a very high serum concentration of anti-beta-amyloid antibodies (above 1 mg ml 1). E2 vaccination polarizes the immune response toward the production of the anti-inflammatory cytokine interleukin-4 and does not induce a T cell response to beta-amyloid. Thus, E2-based vaccines are promising candidates for the development of immunotherapy protocols for AD.

Original languageEnglish
Pages (from-to)604-609
Number of pages6
JournalImmunology and Cell Biology
Volume89
Issue number5
DOIs
Publication statusPublished - Jul 2011

Keywords

  • Alzheimer's disease
  • antibody
  • beta-amyloid
  • immunotherapy

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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    Mantile, F., Basile, C., Cicatiello, V., De Falco, D., Caivano, A., De Berardinis, P., & Prisco, A. (2011). A multimeric immunogen for the induction of immune memory to beta-amyloid. Immunology and Cell Biology, 89(5), 604-609. https://doi.org/10.1038/icb.2010.134