A multiple technical approach to the study of apoptotic cell micronuclei

M. Columbaro, P. Gobbi, F. Renò, F. Luchetti, S. Santi, A. Valmori, E. Falcieri

Research output: Contribution to journalArticle

Abstract

Apoptotic micronuclei have been studied, in different cell types, from a morphologic and functional point of view. Conventional electron microscopy, in various staining conditions, selective cytochemistry for DNA, and freeze fracture for the analysis of chromatin fiber organization and size were performed. In situ TdT and Pol I immunofluorescent techniques were carried out to detect double- and single-strand DNA breaking points by confocal laser scanning microscopy. Apoptotic cell ultrathin cryosections were also performed and were analysed by field emission in lens scanning electron microscopy. Double/single strand massively cleaved DNA was detected in micronuclei, with a highly supercoiled, uniformly packed, very dense arrangement.

Original languageEnglish
Pages (from-to)541-548
Number of pages8
JournalScanning
Volume20
Issue number8
Publication statusPublished - Nov 1998

Fingerprint

DNA
deoxyribonucleic acid
Cells
strands
chromatin
staining
Field emission
Electron microscopy
field emission
Lenses
electron microscopy
Microscopic examination
lenses
microscopy
Scanning
Scanning electron microscopy
scanning electron microscopy
scanning
fibers
Fibers

Keywords

  • Apoptosis
  • Electron microscopy
  • Field emission in lens scanning electron microscopy
  • In situ DNA labelling
  • Micronuclei

ASJC Scopus subject areas

  • Instrumentation

Cite this

Columbaro, M., Gobbi, P., Renò, F., Luchetti, F., Santi, S., Valmori, A., & Falcieri, E. (1998). A multiple technical approach to the study of apoptotic cell micronuclei. Scanning, 20(8), 541-548.

A multiple technical approach to the study of apoptotic cell micronuclei. / Columbaro, M.; Gobbi, P.; Renò, F.; Luchetti, F.; Santi, S.; Valmori, A.; Falcieri, E.

In: Scanning, Vol. 20, No. 8, 11.1998, p. 541-548.

Research output: Contribution to journalArticle

Columbaro, M, Gobbi, P, Renò, F, Luchetti, F, Santi, S, Valmori, A & Falcieri, E 1998, 'A multiple technical approach to the study of apoptotic cell micronuclei', Scanning, vol. 20, no. 8, pp. 541-548.
Columbaro M, Gobbi P, Renò F, Luchetti F, Santi S, Valmori A et al. A multiple technical approach to the study of apoptotic cell micronuclei. Scanning. 1998 Nov;20(8):541-548.
Columbaro, M. ; Gobbi, P. ; Renò, F. ; Luchetti, F. ; Santi, S. ; Valmori, A. ; Falcieri, E. / A multiple technical approach to the study of apoptotic cell micronuclei. In: Scanning. 1998 ; Vol. 20, No. 8. pp. 541-548.
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