Abstract
Apoptotic micronuclei have been studied, in different cell types, from a morphologic and functional point of view. Conventional electron microscopy, in various staining conditions, selective cytochemistry for DNA, and freeze fracture for the analysis of chromatin fiber organization and size were performed. In situ TdT and Pol I immunofluorescent techniques were carried out to detect double- and single-strand DNA breaking points by confocal laser scanning microscopy. Apoptotic cell ultrathin cryosections were also performed and were analysed by field emission in lens scanning electron microscopy. Double/single strand massively cleaved DNA was detected in micronuclei, with a highly supercoiled, uniformly packed, very dense arrangement.
| Original language | English |
|---|---|
| Pages (from-to) | 541-548 |
| Number of pages | 8 |
| Journal | Scanning |
| Volume | 20 |
| Issue number | 8 |
| Publication status | Published - Nov 1998 |
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Keywords
- Apoptosis
- Electron microscopy
- Field emission in lens scanning electron microscopy
- In situ DNA labelling
- Micronuclei
ASJC Scopus subject areas
- Instrumentation
Cite this
A multiple technical approach to the study of apoptotic cell micronuclei. / Columbaro, M.; Gobbi, P.; Renò, F.; Luchetti, F.; Santi, S.; Valmori, A.; Falcieri, E.
In: Scanning, Vol. 20, No. 8, 11.1998, p. 541-548.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A multiple technical approach to the study of apoptotic cell micronuclei
AU - Columbaro, M.
AU - Gobbi, P.
AU - Renò, F.
AU - Luchetti, F.
AU - Santi, S.
AU - Valmori, A.
AU - Falcieri, E.
PY - 1998/11
Y1 - 1998/11
N2 - Apoptotic micronuclei have been studied, in different cell types, from a morphologic and functional point of view. Conventional electron microscopy, in various staining conditions, selective cytochemistry for DNA, and freeze fracture for the analysis of chromatin fiber organization and size were performed. In situ TdT and Pol I immunofluorescent techniques were carried out to detect double- and single-strand DNA breaking points by confocal laser scanning microscopy. Apoptotic cell ultrathin cryosections were also performed and were analysed by field emission in lens scanning electron microscopy. Double/single strand massively cleaved DNA was detected in micronuclei, with a highly supercoiled, uniformly packed, very dense arrangement.
AB - Apoptotic micronuclei have been studied, in different cell types, from a morphologic and functional point of view. Conventional electron microscopy, in various staining conditions, selective cytochemistry for DNA, and freeze fracture for the analysis of chromatin fiber organization and size were performed. In situ TdT and Pol I immunofluorescent techniques were carried out to detect double- and single-strand DNA breaking points by confocal laser scanning microscopy. Apoptotic cell ultrathin cryosections were also performed and were analysed by field emission in lens scanning electron microscopy. Double/single strand massively cleaved DNA was detected in micronuclei, with a highly supercoiled, uniformly packed, very dense arrangement.
KW - Apoptosis
KW - Electron microscopy
KW - Field emission in lens scanning electron microscopy
KW - In situ DNA labelling
KW - Micronuclei
UR - http://www.scopus.com/inward/record.url?scp=0032435819&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032435819&partnerID=8YFLogxK
M3 - Article
C2 - 9891939
AN - SCOPUS:0032435819
VL - 20
SP - 541
EP - 548
JO - Scanning
JF - Scanning
SN - 0161-0457
IS - 8
ER -