Abstract
In Staphylococcus epidermidis, ica locus encodes for the synthesis of a polysaccharide intercellular adhesin (slime or biofilm). A multiplex polymerase chain reaction (PCR) for the detection of the five individual genes of ica locus was developed, with the aim to probe the set of genes in a large collection of Staphylococcus epidermidis clinical isolates. Single representative fragments for icaR, icaA, icaD, icaB, and icaC genes were selected. Multiplex PCR was applied to two reference Staphylococcus epidermidis strains [the non-biofilm-forming ATCC 12228 and the biofilm-forming ATCC 35984 (RP62A)] and to 400 clinical isolates of Staphylococcus epidermidis from orthopedic prosthesis associated infections. The gene profile was compared with the phenotypic biofilm-forming ability, evaluated by means of an optimized Congo red agar (CRA) plate test. Among the clinical isolates, 228 (57%) turned out completely ica positive and were biofilm producing. Among the 172 non-bio film-forming strains (43%), 164 (41%) were completely ica negative and 8 strains (2%) harbored all five ica genes. The ica locus thus proves to be a cluster of strictly linked genes, without any evidence of single gene deletion.
| Original language | English |
|---|---|
| Pages (from-to) | 408-413 |
| Number of pages | 6 |
| Journal | Journal of Biomedical Materials Research - Part A |
| Volume | 75 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Oct 14 2005 |
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Keywords
- ica operon
- Multiplex PCR
- Prosthesis-associated infections
- Staphylococcus epidermidis
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
Cite this
A multiplex PCR method for the detection of all five individual genes of ica locus in Staphylococcus epidermidis. A survey on 400 clinical isolates from prosthesis-associated infections. / Arciola, Carla Renata; Gamberini, Simonetta; Campoccia, Davide; Visai, Livia; Speziale, Pietro; Baldassarri, Lucilla; Montanaro, Lucio.
In: Journal of Biomedical Materials Research - Part A, Vol. 75, No. 2, 14.10.2005, p. 408-413.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A multiplex PCR method for the detection of all five individual genes of ica locus in Staphylococcus epidermidis. A survey on 400 clinical isolates from prosthesis-associated infections
AU - Arciola, Carla Renata
AU - Gamberini, Simonetta
AU - Campoccia, Davide
AU - Visai, Livia
AU - Speziale, Pietro
AU - Baldassarri, Lucilla
AU - Montanaro, Lucio
PY - 2005/10/14
Y1 - 2005/10/14
N2 - In Staphylococcus epidermidis, ica locus encodes for the synthesis of a polysaccharide intercellular adhesin (slime or biofilm). A multiplex polymerase chain reaction (PCR) for the detection of the five individual genes of ica locus was developed, with the aim to probe the set of genes in a large collection of Staphylococcus epidermidis clinical isolates. Single representative fragments for icaR, icaA, icaD, icaB, and icaC genes were selected. Multiplex PCR was applied to two reference Staphylococcus epidermidis strains [the non-biofilm-forming ATCC 12228 and the biofilm-forming ATCC 35984 (RP62A)] and to 400 clinical isolates of Staphylococcus epidermidis from orthopedic prosthesis associated infections. The gene profile was compared with the phenotypic biofilm-forming ability, evaluated by means of an optimized Congo red agar (CRA) plate test. Among the clinical isolates, 228 (57%) turned out completely ica positive and were biofilm producing. Among the 172 non-bio film-forming strains (43%), 164 (41%) were completely ica negative and 8 strains (2%) harbored all five ica genes. The ica locus thus proves to be a cluster of strictly linked genes, without any evidence of single gene deletion.
AB - In Staphylococcus epidermidis, ica locus encodes for the synthesis of a polysaccharide intercellular adhesin (slime or biofilm). A multiplex polymerase chain reaction (PCR) for the detection of the five individual genes of ica locus was developed, with the aim to probe the set of genes in a large collection of Staphylococcus epidermidis clinical isolates. Single representative fragments for icaR, icaA, icaD, icaB, and icaC genes were selected. Multiplex PCR was applied to two reference Staphylococcus epidermidis strains [the non-biofilm-forming ATCC 12228 and the biofilm-forming ATCC 35984 (RP62A)] and to 400 clinical isolates of Staphylococcus epidermidis from orthopedic prosthesis associated infections. The gene profile was compared with the phenotypic biofilm-forming ability, evaluated by means of an optimized Congo red agar (CRA) plate test. Among the clinical isolates, 228 (57%) turned out completely ica positive and were biofilm producing. Among the 172 non-bio film-forming strains (43%), 164 (41%) were completely ica negative and 8 strains (2%) harbored all five ica genes. The ica locus thus proves to be a cluster of strictly linked genes, without any evidence of single gene deletion.
KW - ica operon
KW - Multiplex PCR
KW - Prosthesis-associated infections
KW - Staphylococcus epidermidis
UR - http://www.scopus.com/inward/record.url?scp=26844528393&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=26844528393&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.30445
DO - 10.1002/jbm.a.30445
M3 - Article
C2 - 16088896
AN - SCOPUS:26844528393
VL - 75
SP - 408
EP - 413
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 2
ER -