A multiplex PCR protocol for rapid identification of Candida glabrata and its phylogenetically related species Candida nivariensis and Candida bracarensis

Orazio Romeo, Fabio Scordino, Ida Pernice, Carla Lo Passo, Giuseppe Criseo

Research output: Contribution to journalArticlepeer-review

Abstract

We have developed a multiplex PCR protocol for the detection of Candida glabrata and its closely related species Candida nivariensis and Candida bracarensis. The method uses four PCR primers, targeting the ITS1 region and the 5.8S ribosomal RNA gene. The combination of these primers yielded unique results to all Candida species tested. The PCR assay we developed was found to be a rapid, specific and easy to perform method and it will be useful for characterizing large numbers of isolates for epidemiological studies.

Original languageEnglish
Pages (from-to)117-120
Number of pages4
JournalJournal of Microbiological Methods
Volume79
Issue number1
DOIs
Publication statusPublished - Oct 2009

Keywords

  • Candida bracarensis
  • Candida glabrata
  • Candida nivariensis
  • Multiplex PCR
  • Pathogenic yeasts
  • rDNA

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology
  • Microbiology (medical)

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