A multiresidue analytical method using solid-phase extraction and high-pressure liquid chromatography tandem mass spectrometry to measure pharmaceuticals of different therapeutic classes in urban wastewaters

Sara Castiglioni, Renzo Bagnati, Davide Calamari, Roberto Fanelli, Ettore Zuccato

Research output: Contribution to journalArticle

Abstract

An analytical method with two extraction steps has been developed and validated for the simultaneous determination of 30 pharmaceuticals belonging to various therapeutic categories in urban wastewater. The aim was to boost the little available information on drugs' fates in sewage treatment plants (STPs) and in the receiving surface water. Aqueous samples were divided into two aliquots, each extracted by a different solid-phase extraction (SPE) method and analysed by reversed-phase liquid chromatography tandem mass spectrometry (HPLC-MS-MS). Recoveries of the pharmaceuticals were mostly greater than 70% and the overall variability of the method was below 8%. The instrumental quantification limit (IQL) varied between 30 and 400 pg injected, and the limits of quantification (LOQ) were in the low ng/L range. Nineteen pharmaceuticals were detected in concentrations between 0.5 and 2000 ng/L in effluents collected from several STPs in Italy. Atenolol, ciprofloxacin, furosemide, hydrochlorothiazide, ofloxacin, ranitidine and sulphamethoxazole were the most abundant compounds. The present analytical method was useful to check for pharmaceuticals in various Italian STPs and to identify the most abundant compounds.

Original languageEnglish
Pages (from-to)206-215
Number of pages10
JournalJournal of Chromatography A
Volume1092
Issue number2
DOIs
Publication statusPublished - Oct 28 2005

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High pressure liquid chromatography
Solid Phase Extraction
Waste Water
Tandem Mass Spectrometry
Mass spectrometry
Wastewater
Sewage treatment plants
High Pressure Liquid Chromatography
Sewage
Pharmaceutical Preparations
Therapeutics
Sulfamethoxazole
Ranitidine
Hydrochlorothiazide
Ofloxacin
Atenolol
Liquid chromatography
Furosemide
Reverse-Phase Chromatography
Ciprofloxacin

Keywords

  • High-pressure liquid chromatography tandem mass spectrometry (HPLC-MS-MS)
  • Pharmaceuticals
  • Sewage treatment plant (STP)
  • Urban wastewaters

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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abstract = "An analytical method with two extraction steps has been developed and validated for the simultaneous determination of 30 pharmaceuticals belonging to various therapeutic categories in urban wastewater. The aim was to boost the little available information on drugs' fates in sewage treatment plants (STPs) and in the receiving surface water. Aqueous samples were divided into two aliquots, each extracted by a different solid-phase extraction (SPE) method and analysed by reversed-phase liquid chromatography tandem mass spectrometry (HPLC-MS-MS). Recoveries of the pharmaceuticals were mostly greater than 70{\%} and the overall variability of the method was below 8{\%}. The instrumental quantification limit (IQL) varied between 30 and 400 pg injected, and the limits of quantification (LOQ) were in the low ng/L range. Nineteen pharmaceuticals were detected in concentrations between 0.5 and 2000 ng/L in effluents collected from several STPs in Italy. Atenolol, ciprofloxacin, furosemide, hydrochlorothiazide, ofloxacin, ranitidine and sulphamethoxazole were the most abundant compounds. The present analytical method was useful to check for pharmaceuticals in various Italian STPs and to identify the most abundant compounds.",
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AU - Castiglioni, Sara

AU - Bagnati, Renzo

AU - Calamari, Davide

AU - Fanelli, Roberto

AU - Zuccato, Ettore

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