Abstract
We have produced and characterized a novel murine monoclonal antibody (LAM7) of IgG1 isotype which appears specific for peripheral blood monocytes (PBM) on the basis of histochemical and functional studies. By indirect immunofluorescence, including FACS analysis, the antibody reacts with 90 ± 6% of PBM and with monocytic leukemias, while it is totally unreactive with B and T lymphocytes, platelets, granulocytes, peripheral macrophages, dendritic cells, large granular lymphocytes, and nonmonocytic leukemias. The antigen-presenting capacity of peripheral blood mononuclear cells is abolished by treatment with MoAb LAM7 in an antiglobulin-complement-mediated cytotoxicity test, and restored by addition of purified PBM. The progressive disappearance of the antigen recognized by LAM7 from PBM within approximately 3 days in culture, and its absence from both bone marrow precursors and tissue macrophages, define it as a line-specific and stage-specific differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 310-322 |
| Number of pages | 13 |
| Journal | Clinical Immunology and Immunopathology |
| Volume | 45 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1987 |
Fingerprint
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Pathology and Forensic Medicine
Cite this
A murine monoclonal antibody strictly reactive with human peripheral blood monocytes. / Cuomo, Margherita; Santoro, Luciana; Mottolese, Marcella; Tecce, Raffaele; Rosato, Vittorio; Alescio, Tommaso; Siccardi, Antonio G.; Natali, Pier Giorgio.
In: Clinical Immunology and Immunopathology, Vol. 45, No. 3, 1987, p. 310-322.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A murine monoclonal antibody strictly reactive with human peripheral blood monocytes
AU - Cuomo, Margherita
AU - Santoro, Luciana
AU - Mottolese, Marcella
AU - Tecce, Raffaele
AU - Rosato, Vittorio
AU - Alescio, Tommaso
AU - Siccardi, Antonio G.
AU - Natali, Pier Giorgio
PY - 1987
Y1 - 1987
N2 - We have produced and characterized a novel murine monoclonal antibody (LAM7) of IgG1 isotype which appears specific for peripheral blood monocytes (PBM) on the basis of histochemical and functional studies. By indirect immunofluorescence, including FACS analysis, the antibody reacts with 90 ± 6% of PBM and with monocytic leukemias, while it is totally unreactive with B and T lymphocytes, platelets, granulocytes, peripheral macrophages, dendritic cells, large granular lymphocytes, and nonmonocytic leukemias. The antigen-presenting capacity of peripheral blood mononuclear cells is abolished by treatment with MoAb LAM7 in an antiglobulin-complement-mediated cytotoxicity test, and restored by addition of purified PBM. The progressive disappearance of the antigen recognized by LAM7 from PBM within approximately 3 days in culture, and its absence from both bone marrow precursors and tissue macrophages, define it as a line-specific and stage-specific differentiation.
AB - We have produced and characterized a novel murine monoclonal antibody (LAM7) of IgG1 isotype which appears specific for peripheral blood monocytes (PBM) on the basis of histochemical and functional studies. By indirect immunofluorescence, including FACS analysis, the antibody reacts with 90 ± 6% of PBM and with monocytic leukemias, while it is totally unreactive with B and T lymphocytes, platelets, granulocytes, peripheral macrophages, dendritic cells, large granular lymphocytes, and nonmonocytic leukemias. The antigen-presenting capacity of peripheral blood mononuclear cells is abolished by treatment with MoAb LAM7 in an antiglobulin-complement-mediated cytotoxicity test, and restored by addition of purified PBM. The progressive disappearance of the antigen recognized by LAM7 from PBM within approximately 3 days in culture, and its absence from both bone marrow precursors and tissue macrophages, define it as a line-specific and stage-specific differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0023483218&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023483218&partnerID=8YFLogxK
U2 - 10.1016/0090-1229(87)90084-5
DO - 10.1016/0090-1229(87)90084-5
M3 - Article
C2 - 2445509
AN - SCOPUS:0023483218
VL - 45
SP - 310
EP - 322
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
SN - 0090-1229
IS - 3
ER -