A murine monoclonal antibody strictly reactive with human peripheral blood monocytes

Margherita Cuomo, Luciana Santoro, Marcella Mottolese, Raffaele Tecce, Vittorio Rosato, Tommaso Alescio, Antonio G. Siccardi, Pier Giorgio Natali

Research output: Contribution to journalArticle

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Abstract

We have produced and characterized a novel murine monoclonal antibody (LAM7) of IgG1 isotype which appears specific for peripheral blood monocytes (PBM) on the basis of histochemical and functional studies. By indirect immunofluorescence, including FACS analysis, the antibody reacts with 90 ± 6% of PBM and with monocytic leukemias, while it is totally unreactive with B and T lymphocytes, platelets, granulocytes, peripheral macrophages, dendritic cells, large granular lymphocytes, and nonmonocytic leukemias. The antigen-presenting capacity of peripheral blood mononuclear cells is abolished by treatment with MoAb LAM7 in an antiglobulin-complement-mediated cytotoxicity test, and restored by addition of purified PBM. The progressive disappearance of the antigen recognized by LAM7 from PBM within approximately 3 days in culture, and its absence from both bone marrow precursors and tissue macrophages, define it as a line-specific and stage-specific differentiation.

Original languageEnglish
Pages (from-to)310-322
Number of pages13
JournalClinical Immunology and Immunopathology
Volume45
Issue number3
DOIs
Publication statusPublished - 1987

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Monocytes
Monoclonal Antibodies
Large Granular Lymphocytic Leukemia
Macrophages
Antigens
Indirect Fluorescent Antibody Technique
Granulocytes
Dendritic Cells
Anti-Idiotypic Antibodies
Blood Cells
Leukemia
B-Lymphocytes
Blood Platelets
Immunoglobulin G
Bone Marrow
T-Lymphocytes
Bone and Bones
Antibodies
Therapeutics

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

A murine monoclonal antibody strictly reactive with human peripheral blood monocytes. / Cuomo, Margherita; Santoro, Luciana; Mottolese, Marcella; Tecce, Raffaele; Rosato, Vittorio; Alescio, Tommaso; Siccardi, Antonio G.; Natali, Pier Giorgio.

In: Clinical Immunology and Immunopathology, Vol. 45, No. 3, 1987, p. 310-322.

Research output: Contribution to journalArticle

Cuomo, M, Santoro, L, Mottolese, M, Tecce, R, Rosato, V, Alescio, T, Siccardi, AG & Natali, PG 1987, 'A murine monoclonal antibody strictly reactive with human peripheral blood monocytes', Clinical Immunology and Immunopathology, vol. 45, no. 3, pp. 310-322. https://doi.org/10.1016/0090-1229(87)90084-5
Cuomo, Margherita ; Santoro, Luciana ; Mottolese, Marcella ; Tecce, Raffaele ; Rosato, Vittorio ; Alescio, Tommaso ; Siccardi, Antonio G. ; Natali, Pier Giorgio. / A murine monoclonal antibody strictly reactive with human peripheral blood monocytes. In: Clinical Immunology and Immunopathology. 1987 ; Vol. 45, No. 3. pp. 310-322.
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AU - Cuomo, Margherita

AU - Santoro, Luciana

AU - Mottolese, Marcella

AU - Tecce, Raffaele

AU - Rosato, Vittorio

AU - Alescio, Tommaso

AU - Siccardi, Antonio G.

AU - Natali, Pier Giorgio

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N2 - We have produced and characterized a novel murine monoclonal antibody (LAM7) of IgG1 isotype which appears specific for peripheral blood monocytes (PBM) on the basis of histochemical and functional studies. By indirect immunofluorescence, including FACS analysis, the antibody reacts with 90 ± 6% of PBM and with monocytic leukemias, while it is totally unreactive with B and T lymphocytes, platelets, granulocytes, peripheral macrophages, dendritic cells, large granular lymphocytes, and nonmonocytic leukemias. The antigen-presenting capacity of peripheral blood mononuclear cells is abolished by treatment with MoAb LAM7 in an antiglobulin-complement-mediated cytotoxicity test, and restored by addition of purified PBM. The progressive disappearance of the antigen recognized by LAM7 from PBM within approximately 3 days in culture, and its absence from both bone marrow precursors and tissue macrophages, define it as a line-specific and stage-specific differentiation.

AB - We have produced and characterized a novel murine monoclonal antibody (LAM7) of IgG1 isotype which appears specific for peripheral blood monocytes (PBM) on the basis of histochemical and functional studies. By indirect immunofluorescence, including FACS analysis, the antibody reacts with 90 ± 6% of PBM and with monocytic leukemias, while it is totally unreactive with B and T lymphocytes, platelets, granulocytes, peripheral macrophages, dendritic cells, large granular lymphocytes, and nonmonocytic leukemias. The antigen-presenting capacity of peripheral blood mononuclear cells is abolished by treatment with MoAb LAM7 in an antiglobulin-complement-mediated cytotoxicity test, and restored by addition of purified PBM. The progressive disappearance of the antigen recognized by LAM7 from PBM within approximately 3 days in culture, and its absence from both bone marrow precursors and tissue macrophages, define it as a line-specific and stage-specific differentiation.

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