A murine monoclonal antibody strictly reactive with human peripheral blood monocytes

Margherita Cuomo, Luciana Santoro, Marcella Mottolese, Raffaele Tecce, Vittorio Rosato, Tommaso Alescio, Antonio G. Siccardi, Pier Giorgio Natali

Research output: Contribution to journalArticlepeer-review

Abstract

We have produced and characterized a novel murine monoclonal antibody (LAM7) of IgG1 isotype which appears specific for peripheral blood monocytes (PBM) on the basis of histochemical and functional studies. By indirect immunofluorescence, including FACS analysis, the antibody reacts with 90 ± 6% of PBM and with monocytic leukemias, while it is totally unreactive with B and T lymphocytes, platelets, granulocytes, peripheral macrophages, dendritic cells, large granular lymphocytes, and nonmonocytic leukemias. The antigen-presenting capacity of peripheral blood mononuclear cells is abolished by treatment with MoAb LAM7 in an antiglobulin-complement-mediated cytotoxicity test, and restored by addition of purified PBM. The progressive disappearance of the antigen recognized by LAM7 from PBM within approximately 3 days in culture, and its absence from both bone marrow precursors and tissue macrophages, define it as a line-specific and stage-specific differentiation.

Original languageEnglish
Pages (from-to)310-322
Number of pages13
JournalClinical Immunology and Immunopathology
Volume45
Issue number3
DOIs
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

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