A murine monoclonal antibody strictly reactive with human peripheral blood monocytes

Margherita Cuomo; Luciana Santoro; Marcella Mottolese; Raffaele Tecce; Vittorio Rosato; Tommaso Alescio; Antonio G. Siccardi; Pier Giorgio Natali

doi:10.1016/0090-1229(87)90084-5

A murine monoclonal antibody strictly reactive with human peripheral blood monocytes

Margherita Cuomo, Luciana Santoro, Marcella Mottolese, Raffaele Tecce, Vittorio Rosato, Tommaso Alescio, Antonio G. Siccardi, Pier Giorgio Natali

Istituto Regina Elena

Research output: Contribution to journal › Article › peer-review

Abstract

We have produced and characterized a novel murine monoclonal antibody (LAM7) of IgG₁ isotype which appears specific for peripheral blood monocytes (PBM) on the basis of histochemical and functional studies. By indirect immunofluorescence, including FACS analysis, the antibody reacts with 90 ± 6% of PBM and with monocytic leukemias, while it is totally unreactive with B and T lymphocytes, platelets, granulocytes, peripheral macrophages, dendritic cells, large granular lymphocytes, and nonmonocytic leukemias. The antigen-presenting capacity of peripheral blood mononuclear cells is abolished by treatment with MoAb LAM7 in an antiglobulin-complement-mediated cytotoxicity test, and restored by addition of purified PBM. The progressive disappearance of the antigen recognized by LAM7 from PBM within approximately 3 days in culture, and its absence from both bone marrow precursors and tissue macrophages, define it as a line-specific and stage-specific differentiation.

Original language	English
Pages (from-to)	310-322
Number of pages	13
Journal	Clinical Immunology and Immunopathology
Volume	45
Issue number	3
DOIs	https://doi.org/10.1016/0090-1229(87)90084-5
Publication status	Published - 1987

ASJC Scopus subject areas

Immunology
Immunology and Allergy
Pathology and Forensic Medicine

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A murine monoclonal antibody strictly reactive with human peripheral blood monocytes. / Cuomo, Margherita; Santoro, Luciana; Mottolese, Marcella; Tecce, Raffaele; Rosato, Vittorio; Alescio, Tommaso; Siccardi, Antonio G.; Natali, Pier Giorgio.

In: Clinical Immunology and Immunopathology, Vol. 45, No. 3, 1987, p. 310-322.

Research output: Contribution to journal › Article › peer-review

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title = "A murine monoclonal antibody strictly reactive with human peripheral blood monocytes",

abstract = "We have produced and characterized a novel murine monoclonal antibody (LAM7) of IgG1 isotype which appears specific for peripheral blood monocytes (PBM) on the basis of histochemical and functional studies. By indirect immunofluorescence, including FACS analysis, the antibody reacts with 90 ± 6% of PBM and with monocytic leukemias, while it is totally unreactive with B and T lymphocytes, platelets, granulocytes, peripheral macrophages, dendritic cells, large granular lymphocytes, and nonmonocytic leukemias. The antigen-presenting capacity of peripheral blood mononuclear cells is abolished by treatment with MoAb LAM7 in an antiglobulin-complement-mediated cytotoxicity test, and restored by addition of purified PBM. The progressive disappearance of the antigen recognized by LAM7 from PBM within approximately 3 days in culture, and its absence from both bone marrow precursors and tissue macrophages, define it as a line-specific and stage-specific differentiation.",

author = "Margherita Cuomo and Luciana Santoro and Marcella Mottolese and Raffaele Tecce and Vittorio Rosato and Tommaso Alescio and Siccardi, {Antonio G.} and Natali, {Pier Giorgio}",

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AU - Cuomo, Margherita

AU - Santoro, Luciana

AU - Mottolese, Marcella

AU - Tecce, Raffaele

AU - Rosato, Vittorio

AU - Alescio, Tommaso

AU - Siccardi, Antonio G.

AU - Natali, Pier Giorgio

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N2 - We have produced and characterized a novel murine monoclonal antibody (LAM7) of IgG1 isotype which appears specific for peripheral blood monocytes (PBM) on the basis of histochemical and functional studies. By indirect immunofluorescence, including FACS analysis, the antibody reacts with 90 ± 6% of PBM and with monocytic leukemias, while it is totally unreactive with B and T lymphocytes, platelets, granulocytes, peripheral macrophages, dendritic cells, large granular lymphocytes, and nonmonocytic leukemias. The antigen-presenting capacity of peripheral blood mononuclear cells is abolished by treatment with MoAb LAM7 in an antiglobulin-complement-mediated cytotoxicity test, and restored by addition of purified PBM. The progressive disappearance of the antigen recognized by LAM7 from PBM within approximately 3 days in culture, and its absence from both bone marrow precursors and tissue macrophages, define it as a line-specific and stage-specific differentiation.

AB - We have produced and characterized a novel murine monoclonal antibody (LAM7) of IgG1 isotype which appears specific for peripheral blood monocytes (PBM) on the basis of histochemical and functional studies. By indirect immunofluorescence, including FACS analysis, the antibody reacts with 90 ± 6% of PBM and with monocytic leukemias, while it is totally unreactive with B and T lymphocytes, platelets, granulocytes, peripheral macrophages, dendritic cells, large granular lymphocytes, and nonmonocytic leukemias. The antigen-presenting capacity of peripheral blood mononuclear cells is abolished by treatment with MoAb LAM7 in an antiglobulin-complement-mediated cytotoxicity test, and restored by addition of purified PBM. The progressive disappearance of the antigen recognized by LAM7 from PBM within approximately 3 days in culture, and its absence from both bone marrow precursors and tissue macrophages, define it as a line-specific and stage-specific differentiation.

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