A mutant prion protein sensitizes neurons to glutamate-induced excitotoxicity

Emiliano Biasini, Ursula Unterberger, Isaac H. Solomon, Tania Massignan, Assunta Senatore, Hejiao Bian, Till Voigtlaender, Frederick P. Bowman, Valentina Bonetto, Roberto Chiesa, Jennifer Luebke, Paul Toselli, David A. Harris

Research output: Contribution to journalArticlepeer-review

Abstract

Growing evidence suggests that a physiological activity of the cellular prion protein (PrPC) plays a crucial role in several neurodegenerative disorders, including prion and Alzheimer's diseases. However, how the functional activity of PrPC is subverted to deliver neurotoxic signals remains uncertain. Transgenic (Tg) mice expressing PrP with a deletion of residues 105-125 in the central region (referred to as (ΔCR PrP) provide important insights into this problem. Tg(Δ CR) mice exhibit neonatal lethality and massive degeneration of cerebellar granule neurons, a phenotype that is dose dependently suppressed by the presence of wild-type PrP. When expressed in cultured cells, Δ CR PrP induces large, ionic currents that can be detected by patch-clamping techniques. Here, we tested the hypothesis that abnormal ion channel activity underlies the neuronal death seen in Tg(Δ CR) mice. We find that (Δ CR PrP induces abnormal ionic currents in neurons in culture and in cerebellar slices and that this activity sensitizes the neurons to glutamate-induced, calcium-mediated death. In combination with ultrastructural and biochemical analyses, these results demonstrate a role for glutamate-induced excitotoxicity in PrP-mediated neurodegeneration. A similar mechanism may operate in other neurodegenerative disorders attributable to toxic, β-rich oligomers that bind to PrPC.

Original languageEnglish
Pages (from-to)2408-2418
Number of pages11
JournalJournal of Neuroscience
Volume33
Issue number6
DOIs
Publication statusPublished - Feb 6 2013

ASJC Scopus subject areas

  • Neuroscience(all)

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