A negative cis-acting G-fer element participates in the regulation of expression of the human H-ferritin-encoding gene (FERH)

Renata Barresi, Mario Sirito, Gerard Karsenty, Roberto Ravazzolo

Research output: Contribution to journalArticlepeer-review

Abstract

Ferritin (Fer) is the major iron storage protein in man. Its synthesis is regulated both at the translational and transcriptional levels. In previous studies on transcriptional regulation of the human H-ferritin-encoding gene (FERH), a 160-bp promoter segment was analyzed [Bevilacqua et al., Gene 111 (1992) 255-260]. In order to obtain a more complete view of the elements involved in the transcriptional regulation of FERH, we have studied, in a further upstream region of the human FERH promoter (pFERH), a sequence between -272 and -291, named G-fer, because it contains a stretch of ten G, which binds a nuclear factor present in different cell types. DNA-binding assays and competition experiments suggest that the factor binding to G-fer has binding properties very similar to inhibitory factor-1 (IF-1), an ubiquitous factor that interacts with G-rich elements in the promoters of the mouse type-I collagen genes. DNA transfection experiments in HeLa cells, using either a wild-type or mutated pFERH fused to a reporter gene, showed that a 3-bp substitution mutation, that abolished the binding of the specific factor to G-fer, increased the promoter activity, thus suggesting an inhibitory role for the G-fer element and its cognate trans-acting factor.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalGene
Volume140
Issue number2
DOIs
Publication statusPublished - Mar 25 1994

Keywords

  • collagen regulation, type I
  • DNA-binding factors
  • promoter
  • Transcriptional regulation
  • transcriptional repressor

ASJC Scopus subject areas

  • Genetics

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