A neutralizing monoclonal antibody previously mapped exclusively on human immunodeficiency virus type 1 gp41 recognizes an epitope in p17 sharing the core sequence IEEE

Emanuele Buratti, Sergio G. Tisminetzky, Piero D'Agaro, Francisco E. Baralle

Research output: Contribution to journalArticlepeer-review

Abstract

We report here that a human immunodeficiency virus type 1 (HIV-1)- specific neutralizing monoclonal antibody (MAb 1575) mapped to the conserved putative intracellular region from amino acid residues 735 to 752 (735-752 region) of gp41 also recognizes a region in an extracellular portion of p17. Both epitopes have a core recognition sequence (IEEE) in a nonhomologous context. The IEEE motif found in HIV-1 p17 is located in a region known as HGP-30 (residues 86 to 115) which has been previously associated with virus neutralization, cytotoxic T lymphocyte activity, and mother-to-child transmission. An analysis of available gp41 and p17 sequences demonstrates that in these regions both IEEE sequences are highly conserved in different HIV-1 clades. The presence of the IEEE epitope in p17 allows us to explain some unexpected neutralizing characteristics of MAb 1575. In addition, the gp41 735-752 region has been previously reported both in intra- and extracellular locations. Our results suggest that the extracellular location was the result of cross-reactivity with p17.

Original languageEnglish
Pages (from-to)2457-2462
Number of pages6
JournalJournal of Virology
Volume71
Issue number3
Publication statusPublished - Mar 1997

ASJC Scopus subject areas

  • Immunology

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