A new cytokine (IK) down-regulating HLA class II: Monoclonal antibodies, cloning and chromosome localization

Patricia Krief, Yvette Augery-Bourget, Stephane Plaisance, Marie Françoise Merck, Eric Assier, Valérie Tanchou, Martine Billard, Claude Boucheix, Claude Jasmin, Bruno Azzarone

Research output: Contribution to journalArticle

Abstract

The role of HLA class II Antigens in the control of the immune response is determined not only by the genetic polymorphism of these molecules, but also by their density on the cell surface. It is therefore essential to identify the signals that modulate HLA Class II gene activity in normal and neoplastic cells. We have purified a cytokine (IK factor, 19 kDa) secreted by the leukemic cell line K562 and several cancer cells, which inhibits HLA Class II antigen induction by IFN-gamma. We produced specific mAbs which antagonize the biological effect of IK in colon carcinoma Colo 205 cells induced to express HLA-DR molecules by IFN-gamma. Moreover, in Colo 205, HLA-DR can also be induced by the protein synthesis inhibitor Cycloheximide (0.1 μg ml-1), and addition of IK factor almost completely abolishes HLA class II expression. We have also performed the cloning and the sequencing of a specific cDNA. This probe recognizes a 2.1 Kb mRNA in different cell types. The nucleotide sequence exhibits no homologies with known cytokines. IK gene localization shows that it maps on chromosome 2p15-p14. The transient transfection of the cDNA in COS cells induces the secretion of a biologically active 19 kDa protein which is recognized in Western blot by 1C5B11 blocking mAb. This paper reports the characterization of a new cytokine down-regulating HLA class II Antigens, whose analysis will help to better understand HLA class II gene regulation and the mechanism of escape from immunorecognition in cancer cells.

Original languageEnglish
Pages (from-to)3449-3456
Number of pages8
JournalOncogene
Volume9
Issue number12
Publication statusPublished - Dec 1994

Fingerprint

Organism Cloning
Chromosomes
Monoclonal Antibodies
Cytokines
Histocompatibility Antigens Class II
HLA Antigens
MHC Class II Genes
HLA-DR Antigens
Complementary DNA
Protein Synthesis Inhibitors
COS Cells
Genetic Polymorphisms
Cycloheximide
Transfection
Neoplasms
Colon
Cell Count
Western Blotting
Carcinoma
Cell Line

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Krief, P., Augery-Bourget, Y., Plaisance, S., Merck, M. F., Assier, E., Tanchou, V., ... Azzarone, B. (1994). A new cytokine (IK) down-regulating HLA class II: Monoclonal antibodies, cloning and chromosome localization. Oncogene, 9(12), 3449-3456.

A new cytokine (IK) down-regulating HLA class II : Monoclonal antibodies, cloning and chromosome localization. / Krief, Patricia; Augery-Bourget, Yvette; Plaisance, Stephane; Merck, Marie Françoise; Assier, Eric; Tanchou, Valérie; Billard, Martine; Boucheix, Claude; Jasmin, Claude; Azzarone, Bruno.

In: Oncogene, Vol. 9, No. 12, 12.1994, p. 3449-3456.

Research output: Contribution to journalArticle

Krief, P, Augery-Bourget, Y, Plaisance, S, Merck, MF, Assier, E, Tanchou, V, Billard, M, Boucheix, C, Jasmin, C & Azzarone, B 1994, 'A new cytokine (IK) down-regulating HLA class II: Monoclonal antibodies, cloning and chromosome localization', Oncogene, vol. 9, no. 12, pp. 3449-3456.
Krief P, Augery-Bourget Y, Plaisance S, Merck MF, Assier E, Tanchou V et al. A new cytokine (IK) down-regulating HLA class II: Monoclonal antibodies, cloning and chromosome localization. Oncogene. 1994 Dec;9(12):3449-3456.
Krief, Patricia ; Augery-Bourget, Yvette ; Plaisance, Stephane ; Merck, Marie Françoise ; Assier, Eric ; Tanchou, Valérie ; Billard, Martine ; Boucheix, Claude ; Jasmin, Claude ; Azzarone, Bruno. / A new cytokine (IK) down-regulating HLA class II : Monoclonal antibodies, cloning and chromosome localization. In: Oncogene. 1994 ; Vol. 9, No. 12. pp. 3449-3456.
@article{30547eaabf204527b14dc6aeeac40db5,
title = "A new cytokine (IK) down-regulating HLA class II: Monoclonal antibodies, cloning and chromosome localization",
abstract = "The role of HLA class II Antigens in the control of the immune response is determined not only by the genetic polymorphism of these molecules, but also by their density on the cell surface. It is therefore essential to identify the signals that modulate HLA Class II gene activity in normal and neoplastic cells. We have purified a cytokine (IK factor, 19 kDa) secreted by the leukemic cell line K562 and several cancer cells, which inhibits HLA Class II antigen induction by IFN-gamma. We produced specific mAbs which antagonize the biological effect of IK in colon carcinoma Colo 205 cells induced to express HLA-DR molecules by IFN-gamma. Moreover, in Colo 205, HLA-DR can also be induced by the protein synthesis inhibitor Cycloheximide (0.1 μg ml-1), and addition of IK factor almost completely abolishes HLA class II expression. We have also performed the cloning and the sequencing of a specific cDNA. This probe recognizes a 2.1 Kb mRNA in different cell types. The nucleotide sequence exhibits no homologies with known cytokines. IK gene localization shows that it maps on chromosome 2p15-p14. The transient transfection of the cDNA in COS cells induces the secretion of a biologically active 19 kDa protein which is recognized in Western blot by 1C5B11 blocking mAb. This paper reports the characterization of a new cytokine down-regulating HLA class II Antigens, whose analysis will help to better understand HLA class II gene regulation and the mechanism of escape from immunorecognition in cancer cells.",
author = "Patricia Krief and Yvette Augery-Bourget and Stephane Plaisance and Merck, {Marie Fran{\cc}oise} and Eric Assier and Val{\'e}rie Tanchou and Martine Billard and Claude Boucheix and Claude Jasmin and Bruno Azzarone",
year = "1994",
month = "12",
language = "English",
volume = "9",
pages = "3449--3456",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "12",

}

TY - JOUR

T1 - A new cytokine (IK) down-regulating HLA class II

T2 - Monoclonal antibodies, cloning and chromosome localization

AU - Krief, Patricia

AU - Augery-Bourget, Yvette

AU - Plaisance, Stephane

AU - Merck, Marie Françoise

AU - Assier, Eric

AU - Tanchou, Valérie

AU - Billard, Martine

AU - Boucheix, Claude

AU - Jasmin, Claude

AU - Azzarone, Bruno

PY - 1994/12

Y1 - 1994/12

N2 - The role of HLA class II Antigens in the control of the immune response is determined not only by the genetic polymorphism of these molecules, but also by their density on the cell surface. It is therefore essential to identify the signals that modulate HLA Class II gene activity in normal and neoplastic cells. We have purified a cytokine (IK factor, 19 kDa) secreted by the leukemic cell line K562 and several cancer cells, which inhibits HLA Class II antigen induction by IFN-gamma. We produced specific mAbs which antagonize the biological effect of IK in colon carcinoma Colo 205 cells induced to express HLA-DR molecules by IFN-gamma. Moreover, in Colo 205, HLA-DR can also be induced by the protein synthesis inhibitor Cycloheximide (0.1 μg ml-1), and addition of IK factor almost completely abolishes HLA class II expression. We have also performed the cloning and the sequencing of a specific cDNA. This probe recognizes a 2.1 Kb mRNA in different cell types. The nucleotide sequence exhibits no homologies with known cytokines. IK gene localization shows that it maps on chromosome 2p15-p14. The transient transfection of the cDNA in COS cells induces the secretion of a biologically active 19 kDa protein which is recognized in Western blot by 1C5B11 blocking mAb. This paper reports the characterization of a new cytokine down-regulating HLA class II Antigens, whose analysis will help to better understand HLA class II gene regulation and the mechanism of escape from immunorecognition in cancer cells.

AB - The role of HLA class II Antigens in the control of the immune response is determined not only by the genetic polymorphism of these molecules, but also by their density on the cell surface. It is therefore essential to identify the signals that modulate HLA Class II gene activity in normal and neoplastic cells. We have purified a cytokine (IK factor, 19 kDa) secreted by the leukemic cell line K562 and several cancer cells, which inhibits HLA Class II antigen induction by IFN-gamma. We produced specific mAbs which antagonize the biological effect of IK in colon carcinoma Colo 205 cells induced to express HLA-DR molecules by IFN-gamma. Moreover, in Colo 205, HLA-DR can also be induced by the protein synthesis inhibitor Cycloheximide (0.1 μg ml-1), and addition of IK factor almost completely abolishes HLA class II expression. We have also performed the cloning and the sequencing of a specific cDNA. This probe recognizes a 2.1 Kb mRNA in different cell types. The nucleotide sequence exhibits no homologies with known cytokines. IK gene localization shows that it maps on chromosome 2p15-p14. The transient transfection of the cDNA in COS cells induces the secretion of a biologically active 19 kDa protein which is recognized in Western blot by 1C5B11 blocking mAb. This paper reports the characterization of a new cytokine down-regulating HLA class II Antigens, whose analysis will help to better understand HLA class II gene regulation and the mechanism of escape from immunorecognition in cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=0027973519&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027973519&partnerID=8YFLogxK

M3 - Article

C2 - 7970704

AN - SCOPUS:0027973519

VL - 9

SP - 3449

EP - 3456

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 12

ER -