A new human highly tumorigenic neuroblastoma cell line with undetectable expression of N-myc

P. Cornaglia-Ferraris, M. Ponzoni, P. Montaldo, G. L. Mariottini, E. Donti, D. Di Martino, G. P. Tonini

Research output: Contribution to journalArticlepeer-review


A peculiar human cell line (GI-ME-N) derived from the metastatic bone marrow of a 2-yr-old patient with stage IV neuroblastoma (NB) was extensively characterized. Cell-type-specific markers, tumorigenicity in nude mice, morphology, cytogenetics, and amplification/ expression of the N-myc gene were evaluated. All meta-phases presented the typical lp deletion. Surface markers specific for NB cells, vimentin, and neurofilament proteins were all clearly detectable with immunofluorescence and/ or western blot procedures. Moreover, it was found that GI-ME-N cells did not express N-myc oncogene or HLA class 1 antigens, and were not classified as peripheral neuroectodermal tumor cells. However, extremely short latency and survival times, comparable to peripheral neuroectodermal tumor cells, were observed in nude mice grafted with GI-ME-N. In addition, no correlations were. observed in tumorigenicity of N-myc amplified (IMR32) versus unamplified (SK-N-SH GI-ME-N) human NB cell lines in nude mice. We conclude that N-myc amplification/ expression do not correlate with the aggressiveness of human NB in athymic animals, which is not always explained by the peripheral neuroectodermal tumor cell nature of the malignant cells, either.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalPediatric Research
Issue number1
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health


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