A new immunoperoxidase assay for Lolium perenne-specific IgE in serum based on the biotin/avidin system (BAS)

A. Plebani, L. D. Notarangelo, V. Monafo, L. Nespoli, A. G. Ugazio

Research output: Contribution to journalArticle

Abstract

A new solid-phase immunoassay based on the biotin/avidin system (BAS) for measuring serum Lolium perenne (LP)-specific IgE antibody is described. LP-specific IgE was assayed by the BAS assay and RAST for comparison in the sera of thirty-two normal asymptomatic subjects RAST-negative for LP and of twenty-six subjects with hay fever and RAST-positive for LP. The specificity of the BAS assay for LP-specific IgE was demonstrated by absorption experiments. An overall agreement of 91% (53/58) was observed between the BAS and RAST and a high correlation (r=0.87, P

Original languageEnglish
Pages (from-to)373-378
Number of pages6
JournalClinical Allergy
Volume14
Issue number4
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'A new immunoperoxidase assay for Lolium perenne-specific IgE in serum based on the biotin/avidin system (BAS)'. Together they form a unique fingerprint.

  • Cite this