A new LC-MS/MS confirmation method for the determination of 17 drugs of abuse in oral fluid and its application to real samples

Elisa Bassotti, Giuseppe Maria Merone, Annachiara D'Urso, Fabio Savini, Marcello Locatelli, Angela Tartaglia, Paolo Dossetto, Cristian D'Ovidio, Ugo de Grazia

Research output: Contribution to journalArticlepeer-review

Abstract

A new liquid chromatography–tandem mass spectrometry (LC–MS/MS) confirmation method for the direct analysis of 17 drugs starting from 200 μL of diluted oral fluid (OF), in a single chromatographic run, was developed and validated. Cocaine, benzoylecgonine (BEG), cocaethylene, Δ-9-tetrahydrocannabinol (Δ-9-THC), buprenorphine, 6-acetylmorphine (6AM), morphine, codeine, methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), methylenedioxyamphetamine (MDA), 3,4-Methylenedioxy-N-ethylamphetamine (MDE), ketamine, N-methyl-1-(1,3-benzodioxol-5-yl)-2-butanamine (MBDB) were determined in a chromatographic run of 12 min only with no sample pre-treatment, after the addition of 15 different internal standards (ISs). The method met all requirements in terms of linearity, accuracy (precision and trueness), recovery, and stability requested by FDA guidelines. Carry-over and interferences were negligible, as well as the matrix effects. LLOQs are below the limits defined by European guidelines and Italian national laws. The original oral fluid collections are stable at least six months at −20 °C and one week at +4 °C.

Original languageEnglish
Article number110330
JournalForensic Science International
Volume312
DOIs
Publication statusPublished - Jul 2020

Keywords

  • Confirmation method
  • Drugs of abuse
  • Forensic toxicology
  • Hyphenated configuration
  • Oral fluid

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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