A new member of the ribbon-helix-helix transcription factor superfamily from the plant pathogen Xanthomonas axonopodis pv. citri

Mariana Gallo, Elena Ferrari, Tommaso Eliseo, Irene Amata, Thelma A. Pertinhez, Angela M. Katsuyama, Maurizio Paci, Chuck S. Farah, Alberto Spisni, Daniel O. Cicero

Research output: Contribution to journalArticle

Abstract

XACb0070 is an uncharacterized protein coded by the two large plasmids isolated from Xanthomonas axonopodis pv. citri, the agent of citrus canker and responsible for important economical losses in citrus world production. XACb0070 presents sequence homology only with other hypothetical proteins belonging to plant pathogens, none of which have their structure determined. The NMR-derived solution structure reveals this protein is a homodimer in which each monomer presents two domains with different structural and dynamic properties: a folded N-terminal domain with βαα topology which mediates dimerization and a long disordered C-terminal tail. The folded domain shows high structural similarity to the ribbon-helix-helix transcriptional repressors, a family of DNA-binding proteins of conserved 3D fold but low sequence homology: indeed XACb0070 binds DNA. Primary sequence and fold comparison of XACb0070 with other proteins of the ribbon-helix-helix family together with examination of the genes in the vicinity of xacb0070 suggest the protein might be the component of a toxin-antitoxin system.

Original languageEnglish
Pages (from-to)21-31
Number of pages11
JournalJournal of Structural Biology
Volume170
Issue number1
DOIs
Publication statusPublished - Apr 2010

Fingerprint

Xanthomonas axonopodis
Transcription Factors
Citrus
Sequence Homology
Proteins
Antitoxins
DNA-Binding Proteins
Dimerization
Plasmids
DNA
Genes

Keywords

  • Antitoxin
  • Citrus canker
  • DNA-binding protein
  • NMR
  • Ribbon-helix-helix fold

ASJC Scopus subject areas

  • Structural Biology

Cite this

A new member of the ribbon-helix-helix transcription factor superfamily from the plant pathogen Xanthomonas axonopodis pv. citri. / Gallo, Mariana; Ferrari, Elena; Eliseo, Tommaso; Amata, Irene; Pertinhez, Thelma A.; Katsuyama, Angela M.; Paci, Maurizio; Farah, Chuck S.; Spisni, Alberto; Cicero, Daniel O.

In: Journal of Structural Biology, Vol. 170, No. 1, 04.2010, p. 21-31.

Research output: Contribution to journalArticle

Gallo, M, Ferrari, E, Eliseo, T, Amata, I, Pertinhez, TA, Katsuyama, AM, Paci, M, Farah, CS, Spisni, A & Cicero, DO 2010, 'A new member of the ribbon-helix-helix transcription factor superfamily from the plant pathogen Xanthomonas axonopodis pv. citri', Journal of Structural Biology, vol. 170, no. 1, pp. 21-31. https://doi.org/10.1016/j.jsb.2009.12.022
Gallo, Mariana ; Ferrari, Elena ; Eliseo, Tommaso ; Amata, Irene ; Pertinhez, Thelma A. ; Katsuyama, Angela M. ; Paci, Maurizio ; Farah, Chuck S. ; Spisni, Alberto ; Cicero, Daniel O. / A new member of the ribbon-helix-helix transcription factor superfamily from the plant pathogen Xanthomonas axonopodis pv. citri. In: Journal of Structural Biology. 2010 ; Vol. 170, No. 1. pp. 21-31.
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