A new method for assessment of serum-induced damage to E. coli

G. Zabucchi, G. D. Rottini, M. R. Soranzo, F. Tedesco, P. Patriarca

Research output: Contribution to journalArticle

Abstract

A simple and rapid spectrophotometric assay for the kinetic evaluation of serum-induced damage to E. coli is described, based on changes in the optical density (OD) of a bacterial suspension. Exposure of antibody-coated E. coli to human absorbed serum results in a diphasic response, namely an increase in OD, which reaches a maximum at about 17 min and is followed by a progressive decrease in OD until a minimum value is reached after 45 min. The increase and the decrease in OD are related to bacterial death and bacterial lysis, respectively.

Original languageEnglish
Pages (from-to)253-264
Number of pages12
JournalJournal of Immunological Methods
Volume57
Issue number1-3
DOIs
Publication statusPublished - Feb 25 1983

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Escherichia coli
Serum
Suspensions
Antibodies

Keywords

  • Escherichia coli
  • light scattering
  • serum damage to E. coli

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

A new method for assessment of serum-induced damage to E. coli. / Zabucchi, G.; Rottini, G. D.; Soranzo, M. R.; Tedesco, F.; Patriarca, P.

In: Journal of Immunological Methods, Vol. 57, No. 1-3, 25.02.1983, p. 253-264.

Research output: Contribution to journalArticle

Zabucchi, G. ; Rottini, G. D. ; Soranzo, M. R. ; Tedesco, F. ; Patriarca, P. / A new method for assessment of serum-induced damage to E. coli. In: Journal of Immunological Methods. 1983 ; Vol. 57, No. 1-3. pp. 253-264.
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