A new method for assessment of serum-induced damage to E. coli

G. Zabucchi, G. D. Rottini, M. R. Soranzo, F. Tedesco, P. Patriarca

Research output: Contribution to journalArticlepeer-review

Abstract

A simple and rapid spectrophotometric assay for the kinetic evaluation of serum-induced damage to E. coli is described, based on changes in the optical density (OD) of a bacterial suspension. Exposure of antibody-coated E. coli to human absorbed serum results in a diphasic response, namely an increase in OD, which reaches a maximum at about 17 min and is followed by a progressive decrease in OD until a minimum value is reached after 45 min. The increase and the decrease in OD are related to bacterial death and bacterial lysis, respectively.

Original languageEnglish
Pages (from-to)253-264
Number of pages12
JournalJournal of Immunological Methods
Volume57
Issue number1-3
DOIs
Publication statusPublished - Feb 25 1983

Keywords

  • Escherichia coli
  • light scattering
  • serum damage to E. coli

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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