A new method for cryopreserving adipose-derived stem cells: An attractive and suitable large-scale and long-term cell banking technology

Alfredo De Rosa, Francesco De Francesco, Virginia Tirino, Giuseppe A. Ferraro, Vincenzo Desiderio, Francesca Paino, Giuseppe Pirozzi, Francesco D'Andrea, Gianpaolo Papaccio

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Recent studies have shown potential ways for improving stem cell cryopreservation. The major need for autologous stem cell use is a long-term storage: this arises from the humans' hope of future use of their own cells. Therefore, it is important to evaluate the cell potential of vitality and differentiation before and after cryopreservation. Although several studies have shown a long-term preservation of adipose tissue, a few of them focused their attention to stem cells. The aim of this study was to evaluate the fate of cryopreserved stem cells collected from adipose tissue and stored at low a temperature in liquid nitrogen through an optimal cryopreservation solution (using slowly cooling in 6% threalose, 4% dimethyl sulfoxide, and 10% fetal bovine serum) and to develop a novel approach to efficiently preserve adipose-derived stem cells (ASCs) for future clinical applications. Results showed that stem cells, after being thawed, are still capable of differentiation and express all surface antigens detected before storage, confirming the integrity of their biology. In particular, ASCs differentiated into adipocytes, showed diffuse positivity for PPARγ and adiponectin, and were also able to differentiate into endothelial cells without addition of angiogenic factors. Therefore, ASCs can be long-term cryopreserved, and this, due to their great numbers, is an attractive tool for clinical applications as well as of impact for the derived market.

Original languageEnglish
Pages (from-to)659-667
Number of pages9
JournalTissue Engineering - Part C: Methods
Volume15
Issue number4
DOIs
Publication statusPublished - Dec 1 2009

Fingerprint

Stem cells
Stem Cells
Technology
Cryopreservation
Adipose Tissue
Tissue
Peroxisome Proliferator-Activated Receptors
Dimethyl sulfoxide
Angiogenesis Inducing Agents
Adiponectin
Endothelial cells
Liquid nitrogen
Surface Antigens
Antigens
Dimethyl Sulfoxide
Adipocytes
Nitrogen
Endothelial Cells
Cooling
Temperature

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

Cite this

A new method for cryopreserving adipose-derived stem cells : An attractive and suitable large-scale and long-term cell banking technology. / De Rosa, Alfredo; De Francesco, Francesco; Tirino, Virginia; Ferraro, Giuseppe A.; Desiderio, Vincenzo; Paino, Francesca; Pirozzi, Giuseppe; D'Andrea, Francesco; Papaccio, Gianpaolo.

In: Tissue Engineering - Part C: Methods, Vol. 15, No. 4, 01.12.2009, p. 659-667.

Research output: Contribution to journalArticle

De Rosa, A, De Francesco, F, Tirino, V, Ferraro, GA, Desiderio, V, Paino, F, Pirozzi, G, D'Andrea, F & Papaccio, G 2009, 'A new method for cryopreserving adipose-derived stem cells: An attractive and suitable large-scale and long-term cell banking technology', Tissue Engineering - Part C: Methods, vol. 15, no. 4, pp. 659-667. https://doi.org/10.1089/ten.tec.2008.0674
De Rosa, Alfredo ; De Francesco, Francesco ; Tirino, Virginia ; Ferraro, Giuseppe A. ; Desiderio, Vincenzo ; Paino, Francesca ; Pirozzi, Giuseppe ; D'Andrea, Francesco ; Papaccio, Gianpaolo. / A new method for cryopreserving adipose-derived stem cells : An attractive and suitable large-scale and long-term cell banking technology. In: Tissue Engineering - Part C: Methods. 2009 ; Vol. 15, No. 4. pp. 659-667.
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