Abstract
Recent studies have shown potential ways for improving stem cell cryopreservation. The major need for autologous stem cell use is a long-term storage: this arises from the humans' hope of future use of their own cells. Therefore, it is important to evaluate the cell potential of vitality and differentiation before and after cryopreservation. Although several studies have shown a long-term preservation of adipose tissue, a few of them focused their attention to stem cells. The aim of this study was to evaluate the fate of cryopreserved stem cells collected from adipose tissue and stored at low a temperature in liquid nitrogen through an optimal cryopreservation solution (using slowly cooling in 6% threalose, 4% dimethyl sulfoxide, and 10% fetal bovine serum) and to develop a novel approach to efficiently preserve adipose-derived stem cells (ASCs) for future clinical applications. Results showed that stem cells, after being thawed, are still capable of differentiation and express all surface antigens detected before storage, confirming the integrity of their biology. In particular, ASCs differentiated into adipocytes, showed diffuse positivity for PPARγ and adiponectin, and were also able to differentiate into endothelial cells without addition of angiogenic factors. Therefore, ASCs can be long-term cryopreserved, and this, due to their great numbers, is an attractive tool for clinical applications as well as of impact for the derived market.
| Original language | English |
|---|---|
| Pages (from-to) | 659-667 |
| Number of pages | 9 |
| Journal | Tissue Engineering - Part C: Methods |
| Volume | 15 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Dec 1 2009 |
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ASJC Scopus subject areas
- Biomedical Engineering
- Bioengineering
- Medicine (miscellaneous)
Cite this
A new method for cryopreserving adipose-derived stem cells : An attractive and suitable large-scale and long-term cell banking technology. / De Rosa, Alfredo; De Francesco, Francesco; Tirino, Virginia; Ferraro, Giuseppe A.; Desiderio, Vincenzo; Paino, Francesca; Pirozzi, Giuseppe; D'Andrea, Francesco; Papaccio, Gianpaolo.
In: Tissue Engineering - Part C: Methods, Vol. 15, No. 4, 01.12.2009, p. 659-667.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A new method for cryopreserving adipose-derived stem cells
T2 - An attractive and suitable large-scale and long-term cell banking technology
AU - De Rosa, Alfredo
AU - De Francesco, Francesco
AU - Tirino, Virginia
AU - Ferraro, Giuseppe A.
AU - Desiderio, Vincenzo
AU - Paino, Francesca
AU - Pirozzi, Giuseppe
AU - D'Andrea, Francesco
AU - Papaccio, Gianpaolo
PY - 2009/12/1
Y1 - 2009/12/1
N2 - Recent studies have shown potential ways for improving stem cell cryopreservation. The major need for autologous stem cell use is a long-term storage: this arises from the humans' hope of future use of their own cells. Therefore, it is important to evaluate the cell potential of vitality and differentiation before and after cryopreservation. Although several studies have shown a long-term preservation of adipose tissue, a few of them focused their attention to stem cells. The aim of this study was to evaluate the fate of cryopreserved stem cells collected from adipose tissue and stored at low a temperature in liquid nitrogen through an optimal cryopreservation solution (using slowly cooling in 6% threalose, 4% dimethyl sulfoxide, and 10% fetal bovine serum) and to develop a novel approach to efficiently preserve adipose-derived stem cells (ASCs) for future clinical applications. Results showed that stem cells, after being thawed, are still capable of differentiation and express all surface antigens detected before storage, confirming the integrity of their biology. In particular, ASCs differentiated into adipocytes, showed diffuse positivity for PPARγ and adiponectin, and were also able to differentiate into endothelial cells without addition of angiogenic factors. Therefore, ASCs can be long-term cryopreserved, and this, due to their great numbers, is an attractive tool for clinical applications as well as of impact for the derived market.
AB - Recent studies have shown potential ways for improving stem cell cryopreservation. The major need for autologous stem cell use is a long-term storage: this arises from the humans' hope of future use of their own cells. Therefore, it is important to evaluate the cell potential of vitality and differentiation before and after cryopreservation. Although several studies have shown a long-term preservation of adipose tissue, a few of them focused their attention to stem cells. The aim of this study was to evaluate the fate of cryopreserved stem cells collected from adipose tissue and stored at low a temperature in liquid nitrogen through an optimal cryopreservation solution (using slowly cooling in 6% threalose, 4% dimethyl sulfoxide, and 10% fetal bovine serum) and to develop a novel approach to efficiently preserve adipose-derived stem cells (ASCs) for future clinical applications. Results showed that stem cells, after being thawed, are still capable of differentiation and express all surface antigens detected before storage, confirming the integrity of their biology. In particular, ASCs differentiated into adipocytes, showed diffuse positivity for PPARγ and adiponectin, and were also able to differentiate into endothelial cells without addition of angiogenic factors. Therefore, ASCs can be long-term cryopreserved, and this, due to their great numbers, is an attractive tool for clinical applications as well as of impact for the derived market.
UR - http://www.scopus.com/inward/record.url?scp=72249115794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=72249115794&partnerID=8YFLogxK
U2 - 10.1089/ten.tec.2008.0674
DO - 10.1089/ten.tec.2008.0674
M3 - Article
C2 - 19254116
AN - SCOPUS:72249115794
VL - 15
SP - 659
EP - 667
JO - Tissue Engineering - Part C: Methods
JF - Tissue Engineering - Part C: Methods
SN - 1937-3384
IS - 4
ER -