A new phosphorylated polysaccharide for biomedical applications: Generation of 30 scaffolds with osteogenic activity and coating of titanium oxide surfaces

Daniela Pasqui, Elisa Danieli, Gemma Leone, Paola Torricelli, Rolando Barbucci

Research output: Contribution to journalArticlepeer-review

Abstract

A new phosphorylated derivative of carboxymethylcellulose and amidic carboxymethylcellulose containing one phosphate group for each disaccharide unit was synthesized using sodium trimetaphospahte (STMP) as the phosphating agent. The new polysaccharide was characterized by infrared spectroscopy (FT-IR) and the amount of phosphate groups was determined by elemental analysis. These modified polysaccharides were used both to prepare 30 scaffolds and functionalize titanium oxide surfaces with the aim to improve the osseointegration with the host tissue. The presence of phosphate groups modify the physical-chemical properties of the hydrogels with respect to the native ones. The evaluation of the bioactivity of the phosphorylated carboxymethylcellulose hydrogels towards osteoblast-like cells showed a significant increase in the osteocalcin production. The modified surfaces were chemically characterized by means of X-ray photoelectron spectroscopy (XPS) and FT-IR, whereas the surface topography was analysed by Atomic Force Measurements (AFM) measurements before and after the polysaccharide coating. In vitro biological tests using osteoblast-like cells demonstrated that phosphorylated carboxymethylcellulose functionalized TiO 2 surfaces promoted better cell adhesion and significantly enhanced their proliferation. These findings suggest that the phosphate polysaccharide both as a 3D scaffold and as a surface coating promotes osteoblast growth potentially improving the biomaterial osseointegration rate.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalMacromolecular Symposia
Volume266
Issue number1
DOIs
Publication statusPublished - Jun 2008

Keywords

  • 3D scaffolds
  • Biomedical applications
  • Osteogenic activity

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics
  • Condensed Matter Physics

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