A new PRINS technique for a rapid detection of chromosome aneuploidies

Savino Calvano; Giovanni Pio De Cillis; Rosanna Latino; Angelo Notarangelo; Paolo Gasparini; Leopoldo Zelante

A new PRINS technique for a rapid detection of chromosome aneuploidies

Savino Calvano, Giovanni Pio De Cillis, Rosanna Latino, Angelo Notarangelo, Paolo Gasparini, Leopoldo Zelante

Research output: Contribution to journal › Article › peer-review

Abstract

The primed in situ labeling (PRINS) technique is an alternative method to the traditional Fluorescent in Situ Hybridization technique (FISH) for chromosome anomalyes identification. We developed a new simplified PRINS protocol and applied it to detect DNA alfoid sequences of chromosomes 13, 14, 15, 17, 18, 21, 22, X and Y, either on meta-phase spreads and interphase nuclei. This modified protocol makes PRINS method more rapid for detecting chromosomal aneuploidies.

Original language	English
Pages (from-to)	99-102
Number of pages	4
Journal	Minerva Biotecnologica
Volume	12
Issue number	2
Publication status	Published - Jun 2000

Keywords

Alfoid sequences
Aneuploidies
PRINS

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Cite this

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title = "A new PRINS technique for a rapid detection of chromosome aneuploidies",

abstract = "The primed in situ labeling (PRINS) technique is an alternative method to the traditional Fluorescent in Situ Hybridization technique (FISH) for chromosome anomalyes identification. We developed a new simplified PRINS protocol and applied it to detect DNA alfoid sequences of chromosomes 13, 14, 15, 17, 18, 21, 22, X and Y, either on meta-phase spreads and interphase nuclei. This modified protocol makes PRINS method more rapid for detecting chromosomal aneuploidies.",

keywords = "Alfoid sequences, Aneuploidies, PRINS",

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year = "2000",

month = jun,

language = "English",

volume = "12",

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T1 - A new PRINS technique for a rapid detection of chromosome aneuploidies

AU - Calvano, Savino

AU - De Cillis, Giovanni Pio

AU - Latino, Rosanna

AU - Notarangelo, Angelo

AU - Gasparini, Paolo

AU - Zelante, Leopoldo

PY - 2000/6

Y1 - 2000/6

N2 - The primed in situ labeling (PRINS) technique is an alternative method to the traditional Fluorescent in Situ Hybridization technique (FISH) for chromosome anomalyes identification. We developed a new simplified PRINS protocol and applied it to detect DNA alfoid sequences of chromosomes 13, 14, 15, 17, 18, 21, 22, X and Y, either on meta-phase spreads and interphase nuclei. This modified protocol makes PRINS method more rapid for detecting chromosomal aneuploidies.

AB - The primed in situ labeling (PRINS) technique is an alternative method to the traditional Fluorescent in Situ Hybridization technique (FISH) for chromosome anomalyes identification. We developed a new simplified PRINS protocol and applied it to detect DNA alfoid sequences of chromosomes 13, 14, 15, 17, 18, 21, 22, X and Y, either on meta-phase spreads and interphase nuclei. This modified protocol makes PRINS method more rapid for detecting chromosomal aneuploidies.

KW - Alfoid sequences

KW - Aneuploidies

KW - PRINS

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M3 - Article

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JO - Minerva Biotecnologica

JF - Minerva Biotecnologica

SN - 1120-4826

IS - 2

ER -

A new PRINS technique for a rapid detection of chromosome aneuploidies

Abstract

Keywords

ASJC Scopus subject areas

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