A new rapid methodological strategy to assess BRCA mutational status

Emilia Vuttariello, Marco Borra, Celeste Calise, Elvira Mauriello, Stefano Greggi, Aldo Vecchione, Elio Biffali, Gennaro Chiappetta

Research output: Contribution to journalArticle

Abstract

Hereditary cancers account for approximately 10 % of breast and ovarian cancers. Mutations of the BRCA1 and BRCA2 genes, encoding two proteins involved in DNA repair, underlie most cases of such hereditary cancers. Women with BRCA mutations develop breast cancer in 50-80 % of cases and ovarian cancer in 10-40 % of cases. Assessing BRCA mutational status is needed to direct the clinical management of women with predisposition to these hereditary cancers. However, BRCA screening constitutes a bottleneck in terms of costs and time to deliver results. We developed a PCR-based assay using 73 primer pairs covering the entire coding regions of BRCA1 and BRCA2. PCR primers, containing at the 5' end the universal M13 primer sequences, were pre-spotted in 96-well plates. Following PCR, direct sequencing was performed using M13 primers, allowing to standardize the conditions. PCR amplification and sequencing were successful for each amplicon. We tested and validated the assay on 10 known gDNAs from patients with Hereditary breast and ovarian cancer (HBOC). Our strategy is a promising time and cost-effective method to detect BRCA mutations in the clinical setting, which is essential to formulate a personalized therapy for patients with HBOC.

Original languageEnglish
Pages (from-to)954-960
Number of pages7
JournalMolecular Biotechnology
Volume54
Issue number3
DOIs
Publication statusPublished - Jul 2013

Keywords

  • BRCA1
  • BRCA2
  • Direct sequencing
  • Genetic testing
  • Hereditary breast and ovarian cancer (HBOC)

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Molecular Biology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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  • Cite this

    Vuttariello, E., Borra, M., Calise, C., Mauriello, E., Greggi, S., Vecchione, A., Biffali, E., & Chiappetta, G. (2013). A new rapid methodological strategy to assess BRCA mutational status. Molecular Biotechnology, 54(3), 954-960. https://doi.org/10.1007/s12033-012-9646-0